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Science 18 December 1992:
Vol. 258. no. 5090, pp. 1932 - 1935
DOI: 10.1126/science.1470915
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Articles

Science, Vol 258, Issue 5090, 1932-1935
Copyright © 1992 by American Association for the Advancement of Science

articles

Cryobiological preservation of Drosophila embryos

P Mazur, KW Cole, JW Hall, PD Schreuders, and AP Mahowald

Biology Division, Oak Ridge National Laboratory, TN 37831-8077.

The inability to cryobiologically preserve the fruit fly Drosophila melanogaster has required that fly stocks be maintained by frequent transfer of adults. This method is costly in terms of time and can lead to loss of stocks. Traditional slow freezing methods do not succeed because the embryos are highly sensitive to chilling. With the procedures described here, 68 percent of precisely staged 15-hour Oregon R (wild-type) embryos hatch after vitrification at -205 degrees C, and 40 percent of the resulting larvae develop into normal adult flies. These embryos are among the most complex organisms successfully preserved by cryobiology.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Effect of Warming Rate on the Survival of Vitrified Mouse Oocytes and on the Recrystallization of Intracellular Ice.
S. Seki and P. Mazur (2008)
Biol Reprod 79, 727-737
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Cryopreservation of the Germplasm of Animals Used in Biological and Medical Research: Importance, Impact, Status, and Future Directions.
P. Mazur, S.P Leibo, and G. E Seidel Jr. (2008)
Biol Reprod 78, 2-12
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Novel direct cover vitrification for cryopreservation of ovarian tissues increases follicle viability and pregnancy capability in mice.
S.-U. Chen, C.-L. Chien, M.-Y. Wu, T.-H. Chen, S.-M. Lai, C.-W. Lin, and Y.-S. Yang (2006)
Hum. Reprod. 21, 2794-2800
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Permeation and toxicity of ethylene glycol and methanol in larvae of Anopheles gambiae.
X.-H. Liu, H. Pan, and P. Mazur (2003)
J. Exp. Biol. 206, 2221-2228
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A Genetic Screen for Zygotic Embryonic Lethal Mutations Affecting Cuticular Morphology in the Wasp Nasonia vitripennis.
M. A. Pultz, K. K. Zimmerman, N. M. Alto, M. Kaeberlein, S. K. Lange, J. N. Pitt, N. L. Reeves, and D. L. Zehrung (2000)
Genetics 154, 1213-1229
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Simple, efficient and successful vitrification of bovine blastocysts using electron microscope grids.
S.-P. Park, E. Y. Kim, D. I. Kim, N. H. Park, Y. S. Won, S. H. Yoon, K. S. Chung, and J. H. Lim (1999)
Hum. Reprod. 14, 2838-2843
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INTERSTITIAL CELLULAR AND MATRIX RESTORATION OF CARDIAC VALVES AFTERCRYOPRESERVATION.
R. H. Messier Jr, B. L. Bass, P. W. Domkowski, and R. A. Hopkins (1999)
J. Thorac. Cardiovasc. Surg. 118, 36-49
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