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Science 11 December 1992:
Vol. 258. no. 5089, pp. 1775 - 1780
DOI: 10.1126/science.1465612
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Articles

Science, Vol 258, Issue 5089, 1775-1780
Copyright © 1992 by American Association for the Advancement of Science

articles

Interaction of U6 snRNA with a sequence required for function of the nematode SL RNA in trans-splicing

GJ Hannon, PA Maroney, YT Yu, GE Hannon, and TW Nilsen

Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, OH 44106.

Nematode trans-spliced leader (SL) RNAs are composed of two domains, an exon [the 22-nucleotide spliced leader] and a small nuclear RNA (snRNA)-like sequence. Participation in vitro of the spliced leader RNA in trans-splicing reactions is independent of the exon sequence or size and instead depends on features contained in the snRNA-like domain of the molecule. Chemical modification interference analysis has revealed that two short sequence elements in the snRNA-like domain are necessary for SL RNA activity. These elements are sufficient for such activity because when added to a 72-nucleotide fragment of a nematode U1 snRNA, this hybrid RNA could participate in trans-splicing reactions in vitro. One of the critical sequence elements may function by base-pairing with U6 snRNA, an essential U snRNA for both cis- and trans-splicing.


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