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Science 4 December 1992:
Vol. 258. no. 5088, pp. 1654 - 1658
DOI: 10.1126/science.8966547
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Articles

Science, Vol 258, Issue 5088, 1654-1658
Copyright © 1992 by American Association for the Advancement of Science

articles

Expression of an inward-rectifying potassium channel by the Arabidopsis KAT1 cDNA

DP Schachtman, JI Schroeder, WJ Lucas, JA Anderson, and RF Gaber

Department of Biology, University of California, San Diego, La Jolla, CA 92093, USA.

Inward-rectifying potassium channels located in the plasma membrane of higher plant and animal cells contribute to cellular homeostasis and excitability. The genes encoding this specific class of K+ channels have not been functionally identified. This report shows that a single messenger RNA transcript from the Arabidopsis thaliana KAT1 complementary DNA confers the functional expression of a hyperpolarization-activated K+ channel in Xenopus oocytes. The channels encoded by KAT1 are highly selective for K+ over other monovalent cations, are blocked by tetraethylammonium and barium, and have a single channel conductance of 28 +/- 7 picosiemens with 118 millimolar K+ in the bathing solution. These functional characteristics, typical of inward-rectifying K+ channels in eukaryotic cells, demonstrate that KAT1 encodes an inward-rectifying K+ channel.


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