Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 20 November 1992:
Vol. 258. no. 5086, pp. 1350 - 1353
DOI: 10.1126/science.1455228
Prev | Table of Contents | Next

Articles

Science, Vol 258, Issue 5086, 1350-1353
Copyright © 1992 by American Association for the Advancement of Science

articles

Activation of a plant gene by T-DNA tagging: auxin-independent growth in vitro

H Hayashi, I Czaja, H Lubenow, J Schell, and R Walden

Faculty of Agriculture, University of Tokyo, Japan.

A transferred DNA (T-DNA) tagging vector with the potential to produce dominant mutations was used with cocultured Agrobacterium tumefaciens and protoplasts to tag genes involved in the action of the plant growth substance auxin. Transgenic calli were selected for their ability to grow in the absence of auxin in the culture media. From one experiment, 12 calli that displayed this phenotype were recovered, of which 11 were able to regenerate into plants. In one plant studied in detail, protoplast division in the absence of auxin genetically cosegregated with a single T-DNA insert. A messenger RNA encoded by a 6.4-kilobase sequence of plant genomic DNA rescued from the mutant is overexpressed relative to untransformed plants. The genomic DNA, as well as a cognate complementary DNA, once transfected into protoplasts promote growth and cell division in vitro in the absence of exogenously added auxin.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
An Arabidopsis Purple Acid Phosphatase with Phytase Activity Increases Foliar Ascorbate.
W. Zhang, H. A. Gruszewski, B. I. Chevone, and C. L. Nessler (2008)
Plant Physiology 146, 431-440
   Abstract »    Full Text »    PDF »
Arabidopsis Cotyledon-Specific Chloroplast Biogenesis Factor CYO1 Is a Protein Disulfide Isomerase.
H. Shimada, M. Mochizuki, K. Ogura, J. E. Froehlich, K. W. Osteryoung, Y. Shirano, D. Shibata, S. Masuda, K. Mori, and K.-i. Takamiya (2007)
PLANT CELL 19, 3157-3169
   Abstract »    Full Text »    PDF »
Overexpression of an auxilin-like gene (F9E10.5) can suppress Al uptake in roots of Arabidopsis.
B. Ezaki, H. Kiyohara, H. Matsumoto, and S. Nakashima (2007)
J. Exp. Bot. 58, 497-506
   Abstract »    Full Text »    PDF »
Arabidopsis Mutants by Activation Tagging in which Photosynthesis Genes are Expressed in Dedifferentiated Calli..
Y. Niwa, S. Goto, T. Nakano, M. Sakaiya, T. Hirano, H. Tsukaya, Y. Komeda, and H. Kobayashi (2006)
Plant Cell Physiol. 47, 319-331
   Abstract »    Full Text »    PDF »
Identification of plant stress-responsive determinants in arabidopsis by large-scale forward genetic screens.
H. Koiwa, R. A. Bressan, and P. M. Hasegawa (2006)
J. Exp. Bot. 57, 1119-1128
   Abstract »    Full Text »    PDF »
ACTIVATOR of Spomin::LUC1/WRINKLED1 of Arabidopsis thaliana Transactivates Sugar-inducible Promoters.
T. Masaki, N. Mitsui, H. Tsukagoshi, T. Nishii, A. Morikami, and K. Nakamura (2005)
Plant Cell Physiol. 46, 547-556
   Abstract »    Full Text »    PDF »
Activation of the cytochrome P450 gene, CYP72C1, reduces the levels of active brassinosteroids in vivo.
M. Nakamura, T. Satoh, S.-I. Tanaka, N. Mochizuki, T. Yokota, and A. Nagatani (2005)
J. Exp. Bot. 56, 833-840
   Abstract »    Full Text »    PDF »
Overexpression of Constitutive Differential Growth 1 Gene, Which Encodes a RLCKVII-Subfamily Protein Kinase, Causes Abnormal Differential and Elongation Growth after Organ Differentiation in Arabidopsis.
H. Muto, N. Yabe, T. Asami, K. Hasunuma, and K. T. Yamamoto (2004)
Plant Physiology 136, 3124-3133
   Abstract »    Full Text »    PDF »
Recessive-interfering mutations in the gibberellin signaling gene SLEEPY1 are rescued by overexpression of its homologue, SNEEZY.
L. C. Strader, S. Ritchie, J. D. Soule, K. M. McGinnis, and C. M. Steber (2004)
PNAS 101, 12771-12776
   Abstract »    Full Text »    PDF »
The STT3a Subunit Isoform of the Arabidopsis Oligosaccharyltransferase Controls Adaptive Responses to Salt/Osmotic Stress.
H. Koiwa, F. Li, M. G. McCully, I. Mendoza, N. Koizumi, Y. Manabe, Y. Nakagawa, J. Zhu, A. Rus, J. M. Pardo, et al. (2003)
PLANT CELL 15, 2273-2284
   Abstract »    Full Text »    PDF »
Activation Tagging of a Dominant Gibberellin Catabolism Gene (GA 2-oxidase) from Poplar That Regulates Tree Stature.
V. B. Busov, R. Meilan, D. W. Pearce, C. Ma, S. B. Rood, and S. H. Strauss (2003)
Plant Physiology 132, 1283-1291
   Abstract »    Full Text »    PDF »
T-DNA Insertional Mutagenesis for Activation Tagging in Rice.
D.-H. Jeong, S. An, H.-G. Kang, S. Moon, J.-J. Han, S. Park, H. S. Lee, K. An, and G. An (2002)
Plant Physiology 130, 1636-1644
   Abstract »    Full Text »    PDF »
Large-Scale Structure -Function Analysis of the Arabidopsis RPM1 Disease Resistance Protein.
P. Tornero, R. A. Chao, W. N. Luthin, S. A. Goff, and J. L. Dangl (2002)
PLANT CELL 14, 435-450
   Abstract »    Full Text »    PDF »
Insertional Mutagenesis of Genes Required for Seed Development in Arabidopsis thaliana.
J. McElver, I. Tzafrir, G. Aux, R. Rogers, C. Ashby, K. Smith, C. Thomas, A. Schetter, Q. Zhou, M. A. Cushman, et al. (2001)
Genetics 159, 1751-1763
   Abstract »    Full Text »    PDF »
Cloning of an Arabidopsis Patatin-Like Gene, STURDY, by Activation T-DNA Tagging.
S. Huang, R. E. Cerny, D. S. Bhat, and S. M. Brown (2001)
Plant Physiology 125, 573-584
   Abstract »    Full Text »
The AGAMOUS-LIKE 20 MADS domain protein integrates floral inductive pathways in Arabidopsis.
H. Lee, S.-S. Suh, E. Park, E. Cho, J. H. Ahn, S.-G. Kim, J. S. Lee, Y. M. Kwon, and I. Lee (2000)
Genes & Dev. 14, 2366-2376
   Abstract »    Full Text »
Activation Tagging in Arabidopsis.
D. Weigel, J. H. Ahn, M. A. Blázquez, J. O. Borevitz, S. K. Christensen, C. Fankhauser, C. Ferrándiz, I. Kardailsky, E. J. Malancharuvil, M. M. Neff, et al. (2000)
Plant Physiology 122, 1003-1014
   Abstract »    Full Text »
Activation Tagging of the Floral Inducer FT.
I. Kardailsky, V. K. Shukla, J. H. Ahn, N. Dagenais, S. K. Christensen, J. T. Nguyen, J. Chory, M. J. Harrison, and D. Weigel (1999)
Science 286, 1962-1965
   Abstract »    Full Text »
The Diageotropica Gene Differentially Affects Auxin and Cytokinin Responses throughout Development in Tomato.
C. Coenen and T. L. Lomax (1998)
Plant Physiology 117, 63-72
   Abstract »    Full Text »
Lipochitooligosaccharide-induced tobacco cells release a peptide as mediator of the glycolipid signal.
M. John, J. Schmidt, R. Walden, I. Czaja, M. Dulz, J. Schell, and H. Rohrig (1997)
PNAS 94, 10178-10182
   Abstract »    Full Text »    PDF »
P element insertion-dependent gene activation in the Drosophila eye.
B. A. Hay, R. Maile, and G. M. Rubin (1997)
PNAS 94, 5195-5200
   Abstract »    Full Text »    PDF »
Convergent pathways for lipochitooligosaccharide and auxin signaling in tobacco cells.
H. Rohrig, J. Schmidt, R. Walden, I. Czaja, H. Lubenow, U. Wieneke, J. Schell, and M. John (1996)
PNAS 93, 13389-13392
   Abstract »    Full Text »    PDF »
Growth of Tobacco Protoplasts Stimulated by Synthetic Lipo-Chitooligosaccharides.
H. Rohrig, J. Schmidt, R. Walden, I. Czaja, E. Miklascaroneviccarons, U. Wieneke, J. Schell, and M. John (1995)
Science 269, 841-843
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)