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Science 25 September 1992:
Vol. 257. no. 5078, pp. 1885 - 1894
DOI: 10.1126/science.1411504
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Articles

Science, Vol 257, Issue 5078, 1885-1894
Copyright © 1992 by American Association for the Advancement of Science

articles

Weighing naked proteins: practical, high-accuracy mass measurement of peptides and proteins

BT Chait and SB Kent

Rockefeller University, New York, NY 10021.

Two new technologies have made the study of proteins by mass spectrometry straight-forward. Proteins with molecular masses of up to more than 100 kilodaltons can be analyzed at picomole sensitivities to give simple mass spectra corresponding to the intact molecule. This development has allowed unprecedented accuracy in the determination of the molecular weights of proteins. A number of "case studies" are used to present the revolutionary impact that these powerful new ways of looking at proteins are having on biological research.


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