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Science 28 August 1992:
Vol. 257. no. 5074, pp. 1245 - 1247
DOI: 10.1126/science.1387730
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Articles

Science, Vol 257, Issue 5074, 1245-1247
Copyright © 1992 by American Association for the Advancement of Science

articles

Tentoxin sensitivity of chloroplasts determined by codon 83 of beta subunit of proton-ATPase

A Avni, JD Anderson, N Holland, JD Rochaix, Z Gromet-Elhanan, and M Edelman

Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel.

Tentoxin is a naturally occurring phytotoxic peptide that causes seedling chlorosis and arrests growth in sensitive plants and algae. In vitro, it inhibits activity of the beta subunit of the plastid proton-adenosine triphosphatase (ATPase) from sensitive species. Plastid atpB genes from six closely related, tentoxin-sensitive or -resistant Nicotiana species differ at codon 83, according to their response to the toxin: glutamate correlated with resistance and aspartate correlated with sensitivity. The genetic relevance of this site was confirmed in Chlamydomonas reinhardtii by chloroplast transformation. The alga, normally tentoxin-resistant, was rendered tentoxin-sensitive by mutagenesis of its plastid atpB gene at codon 83. Codon 83 may represent a critical site on the beta subunit that does not compete with nucleotide binding or other catalytic activities.


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