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Science 3 July 1992:
Vol. 257. no. 5066, pp. 96 - 99
DOI: 10.1126/science.1621102
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Articles

Science, Vol 257, Issue 5066, 96-99
Copyright © 1992 by American Association for the Advancement of Science

articles

In vivo calcium elevations in thymocytes with T cell receptors that are specific for self ligands

T Nakayama, Y Ueda, H Yamada, EW Shores, A Singer, and CH June

Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.

Selection of the T cell receptor (TCR) repertoire in the thymus probably involves TCR-mediated signals transduced in developing thymocytes after interaction with thymic stromal cells bearing self ligands. TCR-transduced signals should have identifiable consequences that would distinguish thymocytes whose TCRs have been engaged by self ligands from those whose TCRs have not. Among thymocytes expressing a transgenic TCR of defined specificity, a large number had elevated intracellular calcium concentrations but only when resident in a negatively selecting thymus in which their self ligand was expressed. Thus, developing thymocytes are stimulated by endogenous ligands in vivo to mobilize intracellular calcium, and increased intracellular calcium concentrations may reflect the consequences of intrathymic signaling associated with thymic negative selection.


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