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Science 15 May 1992:
Vol. 256. no. 5059, pp. 1027 - 1030
DOI: 10.1126/science.1317057
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Articles

Science, Vol 256, Issue 5059, 1027-1030
Copyright © 1992 by American Association for the Advancement of Science

articles

Acquisition of myogenic specificity by replacement of three amino acid residues from MyoD into E12

RL Davis and H Weintraub

Howard Hughes Medical Institute Laboratory, Fred Hutchinson Cancer Research Center, Seattle, WA 98104.

The basic helix-loop-helix (bHLH) protein MyoD is a transcription factor that is important for the induction of the myogenic phenotype. The DNA binding basic region (13 amino acids) is necessary for recognition of the consensus MyoD binding site, for transcriptional activation, and for conversion of fibroblasts to muscle. In contrast, the non-tissue-specific bHLH protein E12 can bind to the MyoD binding site but does not induce myogenesis. Here, it is shown that only two amino acids in the MyoD basic region and a single amino acid from the junction, which separates the basic region and helix 1, are sufficient for myogenic specificity when substituted into the corresponding region of E12. These findings suggest that the recognition of particular determinants in the basic region is required for conversion of fibroblasts to muscle.


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