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Science 1 May 1992:
Vol. 256. no. 5057, pp. 659 - 663
DOI: 10.1126/science.1585179
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Articles

Science, Vol 256, Issue 5057, 659-663
Copyright © 1992 by American Association for the Advancement of Science

articles

An origin of DNA replication and a transcription silencer require a common element

DH Rivier and J Rine

Department of Molecular and Cell Biology, University of California, Berkley 94720.

A eukaryotic chromosomal origin of replication was identified in the yeast Saccharomyces cerevisiae. By several criteria, including map position, deletion analysis, and a synthetic form of saturation mutagenesis, the origin co-localized with the HMR-E silencer, which is a DNA element that represses transcription of the adjacent genes. A specific site within the silencer was required for both initiation of chromosomal replication and for repression of transcription. This analysis directly demonstrates that initiation of eukaryotic chromosomal replication is dependent on specific sequence elements and that a particular element can act in both initiation of chromosomal replication and regulation of transcription.


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