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Science 24 April 1992:
Vol. 256. no. 5056, pp. 535 - 538
DOI: 10.1126/science.1373910
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Articles

Science, Vol 256, Issue 5056, 535-538
Copyright © 1992 by American Association for the Advancement of Science

articles

Isolation of a complementary DNA that encodes the mammalian splicing factor SC35

XD Fu and T Maniatis

Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.

The mammalian splicing factor SC35 is required for the first step in the splicing reaction and for spliceosome assembly. The cloning and characterization of a complementary DNA encoding this protein revealed that it is a member of a family of splicing factors that includes mammalian SF2/ASF. This family of proteins is characterized by the presence of a ribonucleoprotein (RNP)-type RNA binding motif and a carboxyl-terminal serine-arginine-rich (SR) domain. A search of the DNA sequence database revealed that the thymus-specific exon (ET) of the c-myb proto-oncogene is encoded on the antisense strand of the SC35 gene.


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SR proteins and splicing control..
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The Ser[IMAGE]-Ser[IMAGE] Pair in HeLa Nuclear Protein p21/SIIR Mediates Ser/Thr Phosphorylation and Is Essential for Rous Sarcoma Virus Long Terminal Repeat Repression.
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The Gene Encoding Human Splicing Factor 9G8.
M. Popielarz, Y. Cavaloc, M.-G. Mattei, R. Gattoni, and J. Stvenin (1995)
J. Biol. Chem. 270, 17830-17835
   Abstract »    Full Text »    PDF »
A Putative Mammalian RNA Helicase with an Arginine-Serine-rich Domain Colocalizes with a Splicing Factor.
J. Sukegawa and Gün. Blobel (1995)
J. Biol. Chem. 270, 15702-15706
   Abstract »    Full Text »    PDF »
Synergistic interactions between two distinct elements of a regulated splicing enhancer..
K W Lynch and T Maniatis (1995)
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   Abstract »    PDF »
Colocalization of a high molecular mass phosphoprotein of the nuclear matrix (p255) with spliceosomes.
S Bisotto, P Lauriault, M Duval, and M Vincent (1995)
J. Cell Sci. 108, 1873-1882
   Abstract »    PDF »
Regulation of alternative splicing in vivo by overexpression of antagonistic splicing factors.
J. Caceres, S Stamm, D. Helfman, and A. Krainer (1994)
Science 265, 1706-1709
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Conserved structures and diversity of functions of RNA-binding proteins.
C. Burd and G Dreyfuss (1994)
Science 265, 615-621
   Abstract »    PDF »
A splicing enhancer exhibits both constitutive and regulated activities..
M Tian and T Maniatis (1994)
Genes & Dev. 8, 1703-1712
   Abstract »    PDF »
The conserved pre-mRNA splicing factor U2AF from Drosophila: requirement for viability.
R Kanaar, S. Roche, E. Beall, M. Green, and D. Rio (1993)
Science 262, 569-573
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Correspondence between a mammalian spliceosome component and an essential yeast splicing factor.
M Bennett and R Reed (1993)
Science 262, 105-108
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Interactions between PRP9 and SPP91 splicing factors identify a protein complex required in prespliceosome assembly..
P Legrain, C Chapon, and F Galisson (1993)
Genes & Dev. 7, 1390-1399
   Abstract »    PDF »
Distinct functions of SR proteins in alternative pre-mRNA splicing.
A. Zahler, K. Neugebauer, W. Lane, and M. Roth (1993)
Science 260, 219-222
   Abstract »    PDF »
Cloning and characterization of PSF, a novel pre-mRNA splicing factor..
J G Patton, E B Porro, J Galceran, P Tempst, and B Nadal-Ginard (1993)
Genes & Dev. 7, 393-406
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Distribution of snRNPs, splicing factor SC-35 and actin in interphase nuclei: immunocytochemical evidence for differential distribution during changes in functional states.
D. Sahlas, K Milankov, P. Park, and U De Boni (1993)
J. Cell Sci. 105, 347-357
   Abstract »    PDF »
Serine-Arginine (SR) Protein-like Factors That Antagonize Authentic SR Proteins and Regulate Alternative Splicing.
A. E. Cowper, J. F. Caceres, A. Mayeda, and G. R. Screaton (2001)
J. Biol. Chem. 276, 48908-48914
   Abstract »    Full Text »    PDF »
Splicing Factors Induce Cystic Fibrosis Transmembrane Regulator Exon 9 Skipping through a Nonevolutionary Conserved Intronic Element.
F. Pagani, E. Buratti, C. Stuani, M. Romano, E. Zuccato, M. Niksic, L. Giglio, D. Faraguna, and F. E. Baralle (2000)
J. Biol. Chem. 275, 21041-21047
   Abstract »    Full Text »    PDF »


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