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Science 24 April 1992:
Vol. 256. no. 5056, pp. 526 - 529
DOI: 10.1126/science.1315076
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Articles

Science, Vol 256, Issue 5056, 526-529
Copyright © 1992 by American Association for the Advancement of Science

articles

Tertiary structure around the guanosine-binding site of the Tetrahymena ribozyme

JF Wang and TR Cech

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309-0215.

A cleavage reagent directed to the active site of the Tetrahymena catalytic RNA was synthesized by derivatization of the guanosine substrate with a metal chelator. When complexed with iron(II), this reagent cleaved the RNA in five regions. Cleavage at adenosine 207, which is far from the guanosine-binding site in the primary and secondary structure, provides a constraint for the higher order folding of the RNA. This cleavage site constitutes physical evidence for a key feature of the Michel-Westhof model. Targeting a reactive entity to a specific site should be generally useful for determining proximity within folded RNA molecules or ribonucleoprotein complexes.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Extraordinarily slow binding of guanosine to the Tetrahymena group I ribozyme: Implications for RNA preorganization and function.
K. Karbstein and D. Herschlag (2003)
PNAS 100, 2300-2305
   Abstract »    Full Text »    PDF »
Movement of the guide sequence during RNA catalysis by a group I ribozyme.
J. Wang, W. Downs, and T. Cech (1993)
Science 260, 504-508
   Abstract »    PDF »


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