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Science 10 April 1992:
Vol. 256. no. 5054, pp. 234 - 237
DOI: 10.1126/science.1566071
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Articles

Science, Vol 256, Issue 5054, 234-237
Copyright © 1992 by American Association for the Advancement of Science

articles

Specific binding of chromosomal protein HMG1 to DNA damaged by the anticancer drug cisplatin

PM Pil and SJ Lippard

Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139.

The mechanism of action of the anticancer compound cis-diamminedichloroplatinum(II) (cisplatin) involves covalent binding to DNA. In an effort to understand the tumor-specific cytotoxicity of such DNA damage, the interactions of these lesions with cellular proteins have been studied. One such protein has been identified as the high-mobility group protein HMG1. Recombinant rat HMG1 binds specifically (dissociation constant 3.7 +/- 2.0 x 10(-7) molar) to DNA containing cisplatin d(GpG) or d(ApG) intrastrand cross-links, which unwind and bend DNA in a specific manner, but not to DNA modified by therapeutically inactive platinum analogs. These results suggest how HMG1 might bind to altered DNA structures and may be helpful in designing new antitumor drugs.


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Characterization of a High Mobility Group 1/2 Homolog in Yeast.
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Synthesis and biological activity of DNA damaging agents that form decoy binding sites for the estrogen receptor.
S. M. Rink, K. J. Yarema, M. S. Solomon, L. A. Paige, B. M. Tadayoni-Rebek, J. M. Essigmann, and R. G. Croy (1996)
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Maize Chromosomal HMGc. TWO CLOSELY RELATED STRUCTURE-SPECIFIC DNA-BINDING PROTEINS SPECIFY A SECOND TYPE OF PLANT HIGH MOBILITY GROUP BOX PROTEIN.
KlausD. Grasser, R. Grimm, and C. Ritt (1996)
J. Biol. Chem. 271, 32900-32906
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Yeast HMG proteins NHP6A/B potentiate promoter-specific transcriptional activation in vivo and assembly of preinitiation complexes in vitro..
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Genes & Dev. 10, 2769-2781
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Cisplatin and Adriamycin Resistance Are Associated with MutLalpha and Mismatch Repair Deficiency in an Ovarian Tumor Cell Line.
J. T. Drummond, A. Anthoney, R. Brown, and P. Modrich (1996)
J. Biol. Chem. 271, 19645-19648
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Human Ku Autoantigen Binds Cisplatin-damaged DNA but Fails to Stimulate Human DNA-activated Protein Kinase.
J. J. Turchi and K. Henkels (1996)
J. Biol. Chem. 271, 13861-13867
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Binding of the Escherichia coli UvrAB Proteins to the DNA Mono- and Diadducts of cis-[N-2-Amino-N-2-methylamino-2,2,1-bicycloheptane]dichloroplatinum(II) and Cisplatin.
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Excision Repair in Mammalian Cells.
A. Sancar and A. Sancar (1995)
J. Biol. Chem. 270, 15915-15918
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Activation of the TFIID-TFIIA complex with HMG-2..
B M Shykind, J Kim, and P A Sharp (1995)
Genes & Dev. 9, 1354-1365
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DNA Looping by Saccharomyces cerevisiae High Mobility Group Proteins NHP6A/B.
T. T. Paull and R. C. Johnson (1995)
J. Biol. Chem. 270, 8744-8754
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Increased DNA-bending Activity and Higher Affinity DNA Binding of High Mobility Group Protein HMG-1 Prepared without Acids.
J. P. Wagner and D. E. Pettijohn (1995)
J. Biol. Chem. 270, 7394-7398
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Mechanisms of DNA excision repair.
A Sancar (1994)
Science 266, 1954-1956
   PDF »
Metal compounds in therapy and diagnosis.
M. Abrams and B. Murrer (1993)
Science 261, 725-730
   Abstract »    PDF »
The nonspecific DNA-binding and -bending proteins HMG1 and HMG2 promote the assembly of complex nucleoprotein structures..
T T Paull, M J Haykinson, and R C Johnson (1993)
Genes & Dev. 7, 1521-1534
   Abstract »    PDF »
Ixr1, a yeast protein that binds to platinated DNA and confers sensitivity to cisplatin.
S. Brown, P. Kellett, and S. Lippard (1993)
Science 261, 603-605
   Abstract »    PDF »
Multiple domains of the RNA polymerase I activator hUBF interact with the TATA-binding protein complex hSL1 to mediate transcription..
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Genes & Dev. 6, 1950-1963
   Abstract »    PDF »
Sequence Specificity, Conformation, and Recognition by HMG1 Protein of Major DNA Interstrand Cross-links of Antitumor Dinuclear Platinum Complexes.
J. Kasparkova, N. Farrell, and V. Brabec (2000)
J. Biol. Chem. 275, 15789-15798
   Abstract »    Full Text »    PDF »
A Role of Basic Residues and the Putative Intercalating Phenylalanine of the HMG-1 Box B in DNA Supercoiling and Binding to Four-way DNA Junctions.
M. Stros and E. Muselikova (2000)
J. Biol. Chem. 275, 35699-35707
   Abstract »    Full Text »    PDF »
Interaction with p53 Enhances Binding of Cisplatin-modified DNA by High Mobility Group 1 Protein.
T. Imamura, H. Izumi, G. Nagatani, T. Ise, M. Nomoto, Y. Iwamoto, and K. Kohno (2001)
J. Biol. Chem. 276, 7534-7540
   Abstract »    Full Text »    PDF »
Interaction of FACT, SSRP1, and the High Mobility Group (HMG) Domain of SSRP1 with DNA Damaged by the Anticancer Drug Cisplatin.
A. T. Yarnell, S. Oh, D. Reinberg, and S. J. Lippard (2001)
J. Biol. Chem. 276, 25736-25741
   Abstract »    Full Text »    PDF »
HMG-D and Histone H1 Interplay during Chromatin Assembly and Early Embryogenesis.
S. S. Ner, T. Blank, M. L. Perez-Paralle, T. A. Grigliatti, P. B. Becker, and A. A. Travers (2001)
J. Biol. Chem. 276, 37569-37576
   Abstract »    Full Text »    PDF »
Transcription-coupled and DNA damage-dependent ubiquitination of RNA polymerase II in vitro.
K.-B. Lee, D. Wang, S. J. Lippard, and P. A. Sharp (2002)
PNAS 99, 4239-4244
   Abstract »    Full Text »    PDF »


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