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Science 10 April 1992:
Vol. 256. no. 5054, pp. 225 - 228
DOI: 10.1126/science.1373522
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Articles

Science, Vol 256, Issue 5054, 225-228
Copyright © 1992 by American Association for the Advancement of Science

articles

Cloning and characterization of inducible nitric oxide synthase from mouse macrophages

QW Xie, HJ Cho, J Calaycay, RA Mumford, KM Swiderek, TD Lee, A Ding, T Troso, and C Nathan

Beatrice and Samuel A. Seaver Laboratory, Department of Medicine, Cornell University Medical College, New York, NY 10021.

Nitric oxide (NO) conveys a variety of messages between cells, including signals for vasorelaxation, neurotransmission, and cytotoxicity. In some endothelial cells and neurons, a constitutive NO synthase is activated transiently by agonists that elevate intracellular calcium concentrations and promote the binding of calmodulin. In contrast, in macrophages, NO synthase activity appears slowly after exposure of the cells to cytokines and bacterial products, is sustained, and functions independently of calcium and calmodulin. A monospecific antibody was used to clone complementary DNA that encoded two isoforms of NO synthase from immunologically activated mouse macrophages. Liquid chromatography-mass spectrometry was used to confirm most of the amino acid sequence. Macrophage NO synthase differs extensively from cerebellar NO synthase. The macrophage enzyme is immunologically induced at the transcriptional level and closely resembles the enzyme in cytokine-treated tumor cells and inflammatory neutrophils.


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Infect. Immun. 69, 6391-6400
   Abstract »    Full Text »    PDF »
Leukocytes infiltrating the pancreatic islets of nonobese diabetic mice are transformed into inactive exiles by combinational anti-cell adhesion therapy.
S. Kommajosyula, S. Reddy, K. Nitschke, J. R. Kanwar, M. Karanam, and G. W. Krissansen (2001)
J. Leukoc. Biol. 70, 510-517
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Identification of Residues Critical for Enzymatic Activity in the Domain Encoded by Exons 8 and 9 of the Human Inducible Nitric Oxide Synthase.
N. T. Eissa, C. M. Haggerty, C. D. Palmer, W. Patton, and J. Moss (2001)
Am. J. Respir. Cell Mol. Biol. 24, 616-620
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Intersection of Interferon and Hypoxia Signal Transduction Pathways in Nitric Oxide-induced Tumor Apoptosis.
D. S. Tendler, C. Bao, T. Wang, E. L. Huang, E. A. Ratovitski, D. A. Pardoll, and C. J. Lowenstein (2001)
Cancer Res. 61, 3682-3688
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Nuclear Factor-kappa B Mediates Simultaneous Induction of Inducible Nitric-Oxide Synthase and Up-Regulation of the Cationic Amino Acid Transporter CAT-2B in Rat Alveolar Macrophages.
R. Hammermann, M. D. M. Dreißig, J. Mössner, M. Fuhrmann, L. Berrino, M. Göthert, and K. Racké (2001)
Mol. Pharmacol. 58, 1294-1302
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Inducible Nitric Oxide Synthase Neutralizes Carbamoylating Potential of 1,3-Bis(2-chloroethyl)-1-nitrosourea in C6 Glioma Cells.
J.-H. Yin, D.-I Yang, H. Chou, E. M. Thompson, J. Xu, and C. Y. Hsu (2001)
J. Pharmacol. Exp. Ther. 297, 308-315
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Down-Regulation of Inducible Nitric-Oxide Synthase (NOS-2) During Parasite-Induced Gut Inflammation: A Path to Identify a Selective NOS-2 Inhibitor.
K. Bian, Y. Harari, M. Zhong, M. Lai, G. Castro, N. Weisbrodt, and F. Murad (2001)
Mol. Pharmacol. 59, 939
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Innate resistance to flavivirus infection in mice controlled by Flv is nitric oxide-independent.
O. J. Silvia, G. R. Shellam, and N. Urosevic (2001)
J. Gen. Virol. 82, 603-607
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IFN-{gamma} + LPS induction of iNOS is modulated by ERK, JNK/SAPK, and p38mapk in a mouse macrophage cell line.
E. D. Chan and D. W. H. Riches (2001)
Am J Physiol Cell Physiol 280, C441-C450
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Inducible nitric oxide synthase: a tissue-specific affair?.
S. J. Wort, J. A. Mitchell, and T. W. Evans (2001)
Am J Physiol Lung Cell Mol Physiol 280, L387-L389
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Role of Nitric Oxide Synthase Type 2 in Acute Infection with Murine Cytomegalovirus.
S. Noda, K. Tanaka, S.-a. Sawamura, M. Sasaki, T. Matsumoto, K. Mikami, Y. Aiba, H. Hasegawa, N. Kawabe, and Y. Koga (2001)
J. Immunol. 166, 3533-3541
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Effect of culture PO2 on macrophage (RAW 264.7) nitric oxide production.
C. M. Otto and J. E. Baumgardner (2001)
Am J Physiol Cell Physiol 280, C280-C287
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Endothelial Nitric Oxide Synthase Gene-Deficient Mice Demonstrate Marked Retardation in Postnatal Bone Formation, Reduced Bone Volume, and Defects in Osteoblast Maturation and Activity.
J. Aguirre, L. Buttery, M. O'Shaughnessy, F. Afzal, I. Fernandez de Marticorena, M. Hukkanen, P. Huang, I. MacIntyre, and J. Polak (2001)
Am. J. Pathol. 158, 247-257
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Activation of Nuclear Factor kappa B and Induction of Inducible Nitric Oxide Synthase by Ureaplasma urealyticum in Macrophages.
Y.-H. Li, Z.-Q. Yan, J. S. Jensen, K. Tullus, and A. Brauner (2000)
Infect. Immun. 68, 7087-7093
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