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Science 27 March 1992:
Vol. 255. no. 5052, pp. 1707 - 1710
DOI: 10.1126/science.1553558

Articles

Science, Vol 255, Issue 5052, 1707-1710
Copyright © 1992 by American Association for the Advancement of Science


articles

Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system

BA Reynolds and S Weiss

Department of Pathology, University of Calgary Faculty of Medicine, Alberta, Canada.

Neurogenesis in the mammalian central nervous system is believed to end in the period just after birth; in the mouse striatum no new neurons are produced after the first few days after birth. In this study, cells isolated from the striatum of the adult mouse brain were induced to proliferate in vitro by epidermal growth factor. The proliferating cells initially expressed nestin, an intermediate filament found in neuroepithelial stem cells, and subsequently developed the morphology and antigenic properties of neurons and astrocytes. Newly generated cells with neuronal morphology were immunoreactive for gamma-aminobutyric acid and substance P, two neurotransmitters of the adult striatum in vivo. Thus, cells of the adult mouse striatum have the capacity to divide and differentiate into neurons and astrocytes.


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PNAS 103, 3875-3880
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Interleukin-23-Expressing Bone Marrow-Derived Neural Stem-Like Cells Exhibit Antitumor Activity against Intracranial Glioma..
X. Yuan, J. Hu, M. L. Belladonna, K. L. Black, and J. S. Yu (2006)
Cancer Res. 66, 2630-2638
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Anchorage-Independent Growth of Mouse Male Germline Stem Cells In Vitro.
M. Kanatsu-Shinohara, K. Inoue, J. Lee, H. Miki, N. Ogonuki, S. Toyokuni, A. Ogura, and T. Shinohara (2006)
Biol Reprod 74, 522-529
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Notch, Epidermal Growth Factor Receptor, and beta1-Integrin Pathways Are Coordinated in Neural Stem Cells.
L. S. Campos, L. Decker, V. Taylor, and W. Skarnes (2006)
J. Biol. Chem. 281, 5300-5309
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Extracellular nucleotide signaling in adult neural stem cells: synergism with growth factor-mediated cellular proliferation.
S. K. Mishra, N. Braun, V. Shukla, M. Fullgrabe, C. Schomerus, H.-W. Korf, C. Gachet, Y. Ikehara, J. Sevigny, S. C. Robson, et al. (2006)
Development 133, 675-684
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Administration of Hematopoietic Cytokines in the Subacute Phase After Cerebral Infarction Is Effective for Functional Recovery Facilitating Proliferation of Intrinsic Neural Stem/Progenitor Cells and Transition of Bone Marrow-Derived Neuronal Cells.
H. Kawada, S. Takizawa, T. Takanashi, Y. Morita, J. Fujita, K. Fukuda, S. Takagi, H. Okano, K. Ando, and T. Hotta (2006)
Circulation 113, 701-710
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Loss of p53 Induces Changes in the Behavior of Subventricular Zone Cells: Implication for the Genesis of Glial Tumors.
S. Gil-Perotin, M. Marin-Husstege, J. Li, M. Soriano-Navarro, F. Zindy, M. F. Roussel, J.-M. Garcia-Verdugo, and P. Casaccia-Bonnefil (2006)
J. Neurosci. 26, 1107-1116
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Retinaldehyde dehydrogenase 2 (RALDH2)-mediated retinoic acid synthesis regulates early mouse embryonic forebrain development by controlling FGF and sonic hedgehog signaling.
V. Ribes, Z. Wang, P. Dolle, and K. Niederreither (2006)
Development 133, 351-361
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p53 suppresses the self-renewal of adult neural stem cells.
K. Meletis, V. Wirta, S.-M. Hede, M. Nister, J. Lundeberg, and J. Frisen (2006)
Development 133, 363-369
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Neurogenesis in the Caudate Nucleus of the Adult Rabbit.
F. Luzzati, S. De Marchis, A. Fasolo, and P. Peretto (2006)
J. Neurosci. 26, 609-621
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