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Science 27 March 1992:
Vol. 255. no. 5052, pp. 1705 - 1707
DOI: 10.1126/science.1553557
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Articles

Science, Vol 255, Issue 5052, 1705-1707
Copyright © 1992 by American Association for the Advancement of Science

articles

An amino acid mutation in a potassium channel that prevents inhibition by protein kinase C

AE Busch, MD Varnum, RA North, and JP Adelman

Vollum Institute, Oregon Health Sciences University, Portland 97201.

A slowly activating, voltage-dependent potassium channel protein cloned from rat kidney was expressed in Xenopus oocytes. Two activators of protein kinase C, 1-oleoyl-2-acetyl-rac-glycerol and phorbol 12,13-didecanoate, inhibited the current. This inhibition was blocked by the kinase inhibitor staurosporine. Inhibition of the current was not seen in channels in which Ser103 was replaced by Ala, although other properties of the current were unchanged. These results indicate that inhibition of the potassium current results from direct phosphorylation of the channel subunit protein at Ser103.


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