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Science 20 March 1992:
Vol. 255. no. 5051, pp. 1570 - 1573
DOI: 10.1126/science.1549783
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Articles

Science, Vol 255, Issue 5051, 1570-1573
Copyright © 1992 by American Association for the Advancement of Science

articles

Structure, expression, and antisense inhibition of the systemin precursor gene

B McGurl, G Pearce, M Orozco-Cardenas, and CA Ryan

Institute of Biological Chemistry, Washington State University, Pullman 99164-6340.

A gene that encodes systemin, a mobile 18-amino acid polypeptide inducer of proteinase inhibitor synthesis in tomato and potato leaves, has been isolated from tomato, Lycopersicon esculentum. Induction of proteinase inhibitors in plants is a response to insect or pathogen attacks. The gene has 10 introns and 11 exons, ten of which are organized as five homologous pairs with an unrelated sequence in the eleventh, encoding systemin. Systemin is proteolytically processed from a 200-amino acid precursor protein, prosystemin. Prosystemin messenger RNA was found in all organs of the plant except the roots and was systemically wound-inducible in leaves. Tomato plants transformed with an antisense prosystemin complementary DNA exhibited greatly suppressed systemic wound induction of proteinase Inhibitor I and II synthesis in leaves.


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Signal perception and transduction in plant defense responses..
Y Yang, J Shah, and D F Klessig (1997)
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   PDF »
Localization and Post-translational Processing of the Wound-induced Leucine Aminopeptidase Proteins of Tomato.
Y.-Q. Gu, W. S. Chao, and L. L. Walling (1996)
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   Abstract »    Full Text »    PDF »
Soybean ENOD40 encodes two peptides that bind to sucrose synthase.
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   Abstract »    Full Text »    PDF »
Distinct roles for jasmonate synthesis and action in the systemic wound response of tomato.
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PNAS 99, 6416-6421
   Abstract »    Full Text »    PDF »
RALF, a 5-kDa ubiquitous polypeptide in plants, arrests root growth and development.
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   Abstract »    Full Text »    PDF »


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