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Science 20 March 1992:
Vol. 255. no. 5051, pp. 1567 - 1570
DOI: 10.1126/science.255.5051.1567
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Articles

Voltage-Dependent Calcium Channels in Plant Vacuoles

OMAR PANTOJA 1, ANGIE GELLI 1, and EDUARDO BLUMWALD 1

1 Centre for Plant Biotechnology, Department of Botany, University of Toronto, Toronto, Ontario, Canada M5S 3B2

Free calcium (Ca2+) in the cytoplasm of plant cells is important for the regulation of many cellular processes and the transduction of stimuli. Control of cytoplasmic Ca2+ involves the activity of pumps, carriers, and possibly ion channels. The patch-clamp technique was used to study Ca2+ channels in the vacuole of sugar beet cells. Vacuolar currents showed inward rectification at negative potentials, with a single-channel conductance of 40 picosiemens and an open probability dependent on potential. Channels were inhibited by verapamil and lanthanum. These channels could participate in the regulation of cytoplasmic Ca2+ by sequestering Ca2+ inside the vacuole.

Submitted on November 22, 1991
Accepted on January 27, 1992


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