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Science 6 March 1992:
Vol. 255. no. 5049, pp. 1261 - 1263
DOI: 10.1126/science.1546328

Articles

Science, Vol 255, Issue 5049, 1261-1263
Copyright © 1992 by American Association for the Advancement of Science


articles

Characterization of peptides bound to the class I MHC molecule HLA-A2.1 by mass spectrometry

DF Hunt, RA Henderson, J Shabanowitz, K Sakaguchi, H Michel, N Sevilir, AL Cox, E Appella, and VH Engelhard

Department of Chemistry, University of Virginia, Charlottesville 22903.

Antigens recognized by T cells are expressed as peptides bound to major histocompatibility complex (MHC) molecules. Microcapillary high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry was used to fractionate and sequence subpicomolar amounts of peptides isolated from the MHC molecule HLA-A2.1. Of 200 different species quantitated, eight were sequenced and four were found in cellular proteins. All were nine residues long and shared a distinct structural motif. The sensitivity and speed of this approach should enhance the analysis of peptides from small quantities of virally infected and transformed cells as well as those associated with autoimmune disease states.


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HLA-A2.1-restricted Education and Cytolytic Activity of CD8+ T Lymphocytes from {beta}2 Microglobulin ({beta}2m) HLA-A2.1 Monochain Transgenic H-2Db {beta}2m Double Knockout Mice.
S. Pascolo, N. Bervas, J. M. Ure, A. G. Smith, F. A. Lemonnier, and B. Perarnau (1997)
J. Exp. Med. 185, 2043-2051
   Abstract »    Full Text »    PDF »
Identification of a Novel Marker for Primordial Smooth Muscle and Its Differential Expression Pattern in Contractile vs Noncontractile Cells.
J. E. Hungerford, J. P. Hoeffler, C. W. Bowers, L. M. Dahm, R. Falchetto, J. Shabanowitz, D. F. Hunt, and C. D. Little (1997)
J. Cell Biol. 137, 925-937
   Abstract »    Full Text »    PDF »
Immediate Early and Early Lytic Cycle Proteins Are Frequent Targets of the Epstein-Barr Virus-induced Cytotoxic T Cell Response.
N.M. Steven, N.E. Annels, A. Kumar, A.M. Leese, M.G. Kurilla, and A.B. Rickinson (1997)
J. Exp. Med. 185, 1605-1618
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Identification of ERF-1 as a member of the AP2 transcription factor family.
L. A. McPherson, V. R. Baichwal, and R. J. Weigel (1997)
PNAS 94, 4342-4347
   Abstract »    Full Text »    PDF »
The 60-kDa Phosphotyrosine Protein in Insulin-treated Adipocytes Is a New Member of the Insulin Receptor Substrate Family.
B. E. Lavan, W. S. Lane, and G. E. Lienhard (1997)
J. Biol. Chem. 272, 11439-11443
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The Immunodominant Antigen of an Ultraviolet-induced Regressor Tumor Is Generated by a Somatic Point Mutation in the DEAD Box Helicase p68.
P. Dubey, R. C. Hendrickson, S. C. Meredith, C. T. Siegel, J. Shabanowitz, J. C.A. Skipper, V. H. Engelhard, D. F. Hunt, and H. Schreiber (1997)
J. Exp. Med. 185, 695-706
   Abstract »    Full Text »    PDF »
HLA Class I Binding Motifs Derived from Random Peptide Libraries Differ at the COOH Terminus from Those of Eluted Peptides.
M. P. Davenport, K. J. Smith, D. Barouch, S. W. Reid, W. M. Bodnar, A. C. Willis, D. F. Hunt, and A. V.S. Hill (1997)
J. Exp. Med. 185, 367-372
   Abstract »    Full Text »    PDF »
The mouse mammary tumor virus env gene is the source of a CD8+ T-cell-stimulating peptide presented by a major histocompatibility complex class I molecule in a murine thymoma.
S. Malarkannan, T. Serwold, V. Nguyen, L. A. Sherman, and N. Shastri (1996)
PNAS 93, 13991-13996
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Identification of the GalNAc Kinase Amino Acid Sequence.
I. Pastuszak, J. O'Donnell, and A. D. Elbein (1996)
J. Biol. Chem. 271, 23653-23656
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Cloning and Functional Characterization of the Gene Encoding the TFIIIB90 Subunit of RNA Polymerase III Transcription Factor TFIIIB.
S. Roberts, S. J. Miller, W. S. Lane, S. Lee, and S. Hahn (1996)
J. Biol. Chem. 271, 14903-14909
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Mimosine Targets Serine Hydroxymethyltransferase.
H.-b. Lin, R. Falchetto, P. J. Mosca, J. Shabanowitz, D. F. Hunt, and J. L. Hamlin (1996)
J. Biol. Chem. 271, 2548-2556
   Abstract »    Full Text »    PDF »
Identification of RANTES, MIP-1alpha, and MIP-1beta as the Major HIV-Suppressive Factors Produced by CD8+ T Cells.
F. Cocchi, A. L. DeVico, A. Garzino-Demo, S. K. Arya, R. C. Gallo, and P. Lusso (1995)
Science 270, 1811-1815
   Abstract »    PDF »
Tolerance to Amino Acid Variations in Peptides Binding to the Major Histocompatibility Complex Class I Protein H-2K^b.
K. Udaka, K.-H. Wiesmüller, S. Kienle, Gün. Jung, and P. Walden (1995)
J. Biol. Chem. 270, 24130-24134
   Abstract »    Full Text »    PDF »
An Ion Pair in Class II Major Histocompatibility Complex Heterodimers Critical for Surface Expression and Peptide Presentation.
E. A. Nalefski, K. T. Y. Shaw, and A. Rao (1995)
J. Biol. Chem. 270, 22351-22360
   Abstract »    Full Text »    PDF »
Human H-Y: a male-specific histocompatibility antigen derived from the SMCY protein.
W Wang, L. Meadows, J. den Haan, N. Sherman, Y Chen, E Blokland, J Shabanowitz, A. Agulnik, R. Hendrickson, C. Bishop, et al. (1995)
Science 269, 1588-1590
   Abstract »    PDF »
Identification of a graft versus host disease-associated human minor histocompatibility antigen.
J. den Haan, N. Sherman, E Blokland, E Huczko, F Koning, J. Drijfhout, J Skipper, J Shabanowitz, D. Hunt, V. Engelhard, et al. (1995)
Science 268, 1476-1480
   Abstract »    PDF »
Major Histocompatibility Class I Molecules Can Present Cryptic Translation Products to T-cells.
N. Shastri, V. Nguyen, and F. Gonzalez (1995)
J. Biol. Chem. 270, 1088-1091
   Abstract »    Full Text »    PDF »
Importance of peptide amino and carboxyl termini to the stability of MHC class I molecules.
M Bouvier and D. Wiley (1994)
Science 265, 398-402
   Abstract »    PDF »
Interaction of MHC class I molecules with the transporter associated with antigen processing.
W. Suh, M. Cohen-Doyle, K Fruh, K Wang, P. Peterson, and D. Williams (1994)
Science 264, 1322-1326
   Abstract »    PDF »
Selecting the T cell receptor repertoire.
M. Bevan, K. Hogquist, and S. Jameson (1994)
Science 264, 796-797
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