Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 28 February 1992:
Vol. 255. no. 5048, pp. 1127 - 1129
DOI: 10.1126/science.1546313
Prev | Table of Contents | Next

Articles

Science, Vol 255, Issue 5048, 1127-1129
Copyright © 1992 by American Association for the Advancement of Science

articles

Isolation of two genes that encode subunits of the yeast transcription factor IIA

JA Ranish, WS Lane, and S Hahn

Fred Hutchinson Cancer Research Center, Seattle, WA 98104.

The yeast transcription factor IIA (TFIIA), a component of the basal transcription machinery of RNA polymerase II and implicated in vitro in regulation of basal transcription, is composed of two subunits of 32 and 13.5 kilodaltons. The genes that encode these subunits, termed TOA1 and TOA2, respectively, were cloned. Neither gene shares obvious sequence similarity with the other or with any other previously identified genes. The recombinant factor bound to a TATA binding protein-DNA complex and complemented yeast and mammalian in vitro transcription systems depleted of TFIIA. Both the TOA1 and TOA2 genes are essential for growth of yeast.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Function and Structural Organization of Mot1 Bound to a Natural Target Promoter.
R. O. Sprouse, I. Shcherbakova, H. Cheng, E. Jamison, M. Brenowitz, and D. T. Auble (2008)
J. Biol. Chem. 283, 24935-24948
   Abstract »    Full Text »    PDF »
Non-optimal TATA Elements Exhibit Diverse Mechanistic Consequences.
J. J. Stewart, J. A. Fischbeck, X. Chen, and L. A. Stargell (2006)
J. Biol. Chem. 281, 22665-22673
   Abstract »    Full Text »    PDF »
Role for Nhp6, Gcn5, and the Swi/Snf Complex in Stimulating Formation of the TATA-Binding Protein-TFIIA-DNA Complex.
D. Biswas, A. N. Imbalzano, P. Eriksson, Y. Yu, and D. J. Stillman (2004)
Mol. Cell. Biol. 24, 8312-8321
   Abstract »    Full Text »    PDF »
Transcriptional Control in Male Germ Cells: General Factor TFIIA Participates in CREM-Dependent Gene Activation.
D. De Cesare, G. M. Fimia, S. Brancorsini, M. Parvinen, and P. Sassone-Corsi (2003)
Mol. Endocrinol. 17, 2554-2565
   Abstract »    Full Text »    PDF »
Structural and Functional Interactions of Transcription Factor (TF) IIA with TFIIE and TFIIF in Transcription Initiation by RNA Polymerase II.
M.-F. Langelier, D. Forget, A. Rojas, Y. Porlier, Z. F. Burton, and B. Coulombe (2001)
J. Biol. Chem. 276, 38652-38657
   Abstract »    Full Text »    PDF »
TFIIA Interacts with TFIID via Association with TATA-Binding Protein and TAF40.
S. M. Kraemer, R. T. Ranallo, R. C. Ogg, and L. A. Stargell (2001)
Mol. Cell. Biol. 21, 1737-1746
   Abstract »    Full Text »
A Testis-specific Transcription Factor IIA (TFIIAtau ) Stimulates TATA-binding Protein-DNA Binding and Transcription Activation.
J. Ozer, P. A. Moore, and P. M. Lieberman (2000)
J. Biol. Chem. 275, 122-128
   Abstract »    Full Text »    PDF »
Analysis of TFIIA Function In Vivo: Evidence for a Role in TATA-Binding Protein Recruitment and Gene-Specific Activation.
Q. Liu, S. E. Gabriel, K. L. Roinick, R. D. Ward, and K. M. Arndt (1999)
Mol. Cell. Biol. 19, 8673-8685
   Abstract »    Full Text »    PDF »
The Karyopherin Kap122p/Pdr6p Imports Both Subunits of the Transcription Factor Iia into the Nucleus.
A. A. Titov and G. Blobel (1999)
J. Cell Biol. 147, 235-246
   Abstract »    Full Text »    PDF »
Nuclear Import of the TATA-binding Protein: Mediation by the Karyopherin Kap114p and a Possible Mechanism for Intranuclear Targeting.
L. F. Pemberton, J. S. Rosenblum, and G. Blobel (1999)
J. Cell Biol. 145, 1407-1417
   Abstract »    Full Text »    PDF »
Identification of a General Transcription Factor TFIIAalpha /beta Homolog Selectively Expressed in Testis.
A. B. Upadhyaya, S. H. Lee, and J. DeJong (1999)
J. Biol. Chem. 274, 18040-18048
   Abstract »    Full Text »    PDF »
Phosphorylation of TFIIA Stimulates TATA Binding Protein-TATA Interaction and Contributes to Maximal Transcription and Viability in Yeast.
S. P. Solow, L. Lezina, and P. M. Lieberman (1999)
Mol. Cell. Biol. 19, 2846-2852
   Abstract »    Full Text »    PDF »
Intermediates in formation and activity of the RNA polymerase II preinitiation complex: holoenzyme recruitment and a postrecruitment role for the TATA box and TFIIB.
J. A. Ranish, N. Yudkovsky, and S. Hahn (1999)
Genes & Dev. 13, 49-63
   Abstract »    Full Text »
Molecular Genetics of the RNA Polymerase II General Transcriptional Machinery.
M. Hampsey (1998)
Microbiol. Mol. Biol. Rev. 62, 465-503
   Abstract »    Full Text »    PDF »
Association of Transcription Factor IIA with TATA Binding Protein Is Required for Transcriptional Activation of a Subset of Promoters and Cell Cycle Progression in Saccharomyces cerevisiae.
J. Ozer, L. E. Lezina, J. Ewing, S. Audi, and P. M. Lieberman (1998)
Mol. Cell. Biol. 18, 2559-2570
   Abstract »    Full Text »
Identification and Analysis of Mot3, a Zinc Finger Protein That Binds to the Retrotransposon Ty Long Terminal Repeat (delta ) in Saccharomyces cerevisiae.
J. M. Madison, A. M. Dudley, and F. Winston (1998)
Mol. Cell. Biol. 18, 1879-1890
   Abstract »    Full Text »
Mechanism of synergy between TATA and initiator: synergistic binding of TFIID following a putative TFIIA-induced isomerization.
K. H. Emami, A. Jain, and S. T. Smale (1997)
Genes & Dev. 11, 3007-3019
   Abstract »    Full Text »    PDF »
Repression of p53-mediated transcription by MDM2: a dual mechanism.
C. J. Thut, J. A. Goodrich, and R. Tjian (1997)
Genes & Dev. 11, 1974-1986
   Abstract »    Full Text »    PDF »
Protease Footprinting Analysis of Ternary Complex Formation by Human TFIIA.
R. Hori and M. Carey (1997)
J. Biol. Chem. 272, 1180-1187
   Abstract »    Full Text »    PDF »
The general transcription factors of RNA polymerase II..
G Orphanides, T Lagrange, and D Reinberg (1996)
Genes & Dev. 10, 2657-2683
   PDF »
Radical mutations reveal TATA-box binding protein surfaces required for activated transcription in vivo..
G O Bryant, L S Martel, S K Burley, and A J Berk (1996)
Genes & Dev. 10, 2491-2504
   Abstract »    PDF »
Transcription Factor IIA Mutations Show Activator-specific Defects and Reveal a IIA Function Distinct from Stimulation of TBP-DNA Binding.
J. Ozer, A. H. Bolden, and P. M. Lieberman (1996)
J. Biol. Chem. 271, 11182-11190
   Abstract »    Full Text »    PDF »
Drosophila TFIIA-S is up-regulated and required during Ras-mediated photoreceptor determination..
M P Zeidler, K Yokomori, R Tjian, and M Mlodzik (1996)
Genes & Dev. 10, 50-59
   Abstract »    PDF »
The TBP-TFIIA interaction in the response to acidic activators in vivo.
L. Stargell and K Struhl (1995)
Science 269, 75-78
   Abstract »    PDF »
Promoter-dependent Photocross-linking of the Acidic Transcriptional Activator E2F-1 to the TATA-binding Protein.
A. Emili and C. J. Ingles (1995)
J. Biol. Chem. 270, 13674-13680
   Abstract »    Full Text »    PDF »
Drosophila TFIIA directs cooperative DNA binding with TBP and mediates transcriptional activation..
K Yokomori, M P Zeidler, J L Chen, C P Verrijzer, M Mlodzik, and R Tjian (1994)
Genes & Dev. 8, 2313-2323
   Abstract »    PDF »
Molecular cloning of the small (gamma) subunit of human TFIIA reveals functions critical for activated transcription..
J Ozer, P A Moore, A H Bolden, A Lee, C A Rosen, and P M Lieberman (1994)
Genes & Dev. 8, 2324-2335
   Abstract »    PDF »
Mot1, a global repressor of RNA polymerase II transcription, inhibits TBP binding to DNA by an ATP-dependent mechanism..
D T Auble, K E Hansen, C G Mueller, W S Lane, J Thorner, and S Hahn (1994)
Genes & Dev. 8, 1920-1934
   Abstract »    PDF »
A mechanism for TAFs in transcriptional activation: activation domain enhancement of TFIID-TFIIA--promoter DNA complex formation..
P M Lieberman and A J Berk (1994)
Genes & Dev. 8, 995-1005
   Abstract »    PDF »
A single cDNA, hTFIIA/alpha, encodes both the p35 and p19 subunits of human TFIIA..
J DeJong and R G Roeder (1993)
Genes & Dev. 7, 2220-2234
   Abstract »    PDF »
Drosophila TFIIA-L is processed into two subunits that are associated with the TBP/TAF complex..
K Yokomori, A Admon, J A Goodrich, J L Chen, and R Tjian (1993)
Genes & Dev. 7, 2235-2245
   Abstract »    PDF »
DNA melting on yeast RNA polymerase II promoters.
C Giardina and J. Lis (1993)
Science 261, 759-762
   Abstract »    PDF »
Identification of a minimal set of proteins that is sufficient for accurate initiation of transcription by RNA polymerase II..
C M Tyree, C P George, L M Lira-DeVito, S L Wampler, M E Dahmus, L Zawel, and J T Kadonaga (1993)
Genes & Dev. 7, 1254-1265
   Abstract »    PDF »
An ATP-dependent inhibitor of TBP binding to DNA..
D T Auble and S Hahn (1993)
Genes & Dev. 7, 844-856
   Abstract »    PDF »
A yeast TFIIB-related factor involved in RNA polymerase III transcription..
T Colbert and S Hahn (1992)
Genes & Dev. 6, 1940-1949
   Abstract »    PDF »
Cloning of the 62-kilodalton component of basic transcription factor BTF2.
L Fischer, M Gerard, C Chalut, Y Lutz, S Humbert, M Kanno, P Chambon, and J. Egly (1992)
Science 257, 1392-1395
   Abstract »    PDF »
TAFII 250 Phosphorylates Human Transcription Factor IIA on Serine Residues Important for TBP Binding and Transcription Activity.
S. Solow, M. Salunek, R. Ryan, and P. M. Lieberman (2001)
J. Biol. Chem. 276, 15886-15892
   Abstract »    Full Text »    PDF »
The Stability of the TFIIA-TBP-DNA Complex Is Dependent on the Sequence of the TATAAA Element.
J. J. Stewart and L. A. Stargell (2001)
J. Biol. Chem. 276, 30078-30084
   Abstract »    Full Text »    PDF »
Fluorescence-based Analyses of the Effects of Full-length Recombinant TAF130p on the Interaction of TATA Box-binding Protein with TATA Box DNA.
U. Banik, J. M. Beechem, E. Klebanow, S. Schroeder, and P. A. Weil (2001)
J. Biol. Chem. 276, 49100-49109
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)