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Science 21 February 1992:
Vol. 255. no. 5047, pp. 996 - 998
DOI: 10.1126/science.1546298
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Articles

Science, Vol 255, Issue 5047, 996-998
Copyright © 1992 by American Association for the Advancement of Science

articles

Reversal of diabetes insipidus in Brattleboro rats: intrahypothalamic injection of vasopressin mRNA

GF Jirikowski, PP Sanna, D Maciejewski-Lenoir, and FE Bloom

Department of Neuropharmacology, Scripps Research Institute, La Jolla, CA 92037.

Messenger RNAs occur within the axons of magnocellular hypothalamic neurons known to secrete oxytocin and vasopressin. In Brattleboro rats, which have a genetic mutation that renders them incapable of vasopressin expression and secretion and thus causes diabetes insipidus, injection into the hypothalamus of purified mRNAs from normal rat hypothalami or of synthetic copies of the vasopressin mRNA leads to selective uptake, retrograde transport, and expression of vasopressin exclusively in the magnocellular neurons. Temporary reversal of their diabetes insipidus (for up to 5 days) can be observed within hours of the injection. Intra-axonal mRNAs may represent an additional category of chemical signals for neurons.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Cellular and Subcellular Evidence for Neuronal Interaction between the Chemokine Stromal Cell-Derived Factor-1/CXCL 12 and Vasopressin: Regulation in the Hypothalamo-Neurohypophysial System of the Brattleboro Rats.
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Endocrinology 149, 310-319
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Ultrastructural Studies of Human Basophils and Mast Cells.
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Modulation of anxiety and neuropeptide Y-Y1 receptors by antisense oligodeoxynucleotides.
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