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Science 14 February 1992:
Vol. 255. no. 5046, pp. 841 - 845
DOI: 10.1126/science.1371365
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Articles

Science, Vol 255, Issue 5046, 841-845
Copyright © 1992 by American Association for the Advancement of Science

articles

Mechanism of transduction by retroviruses

A Swain and JM Coffin

Tufts University School of Medicine, Department of Molecular Biology and Microbiology, Boston, MA 02111.

Retroviruses can capture cellular sequences and express them as oncogenes. Capture has been proposed to be a consequence of the inefficiency of polyadenylation of the viral genome that allows the packaging of cellular sequences flanking the integrated provirus in virions; after transfer into virions, these sequences could be incorporated into the viral genome by illegitimate recombination during reverse transcription. As a test for this hypothesis, a tissue culture system was developed that mimics the transduction process and allows the analysis and quantitation of capture events in a single step. In this model, transduction of sequences adjacent to a provirus depends on the formation of readthrough transcripts and their transmission in virions and leads to various recombinant structures whose formation is independent of sequence similarity at the crossover site. Thus, all events in the transduction process can be attributed to the action of reverse transcriptase on readthrough transcripts without involving deletions of cellular DNA.


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