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Science 14 February 1992:
Vol. 255. no. 5046, pp. 809 - 812
DOI: 10.1126/science.1536005
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Articles

Science, Vol 255, Issue 5046, 809-812
Copyright © 1992 by American Association for the Advancement of Science

articles

The elongation-termination decision in transcription

PH von Hippel and TD Yager

Institute of Molecular Biology, University of Oregon, Eugene.

At any template position, the decision to extend the transcript by one residue or to release the nascent RNA represents a kinetic competition between elongation and termination pathways. This competition is discussed in terms of alternative Eyring transition state barriers; changes in termination efficiency correspond to small changes in the relative heights of these barriers. Elongation complexes are stable at nonterminator positions; a model is presented to explain the destabilization of these complexes at intrinsic termination sites. Functionally analogous effects can operate at rho-dependent terminators. Mechanisms for modulation of termination efficiency by regulatory proteins are described.


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