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Science 24 January 1992:
Vol. 255. no. 5043, pp. 448 - 450
DOI: 10.1126/science.1734521
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Articles

Science, Vol 255, Issue 5043, 448-450
Copyright © 1992 by American Association for the Advancement of Science

articles

Sporogonic development of a malaria parasite in vitro

A Warburg and LH Miller

Malaria Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.

The sporogonic cycle of the avian malaria parasite Plasmodium gallinaceum was completed in vitro. Ookinetes (motile zygotes) were seeded onto a murine basement membrane-like gel (Matrigel) in coculture with Drosophila melanogaster cells (Schneider's L2). Transformation into oocysts as well as subsequent growth and differentiation were observed in parasites attached to Matrigel and depended on the presence of L2 cells. Sporozoites were first observed on day 10 in culture. Specific circumsporozoite protein antigenicity was identified in mature oocysts and in sporozoites. It is now possible to follow the entire life cycle of Plasmodium in vitro.


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