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Science 10 January 1992:
Vol. 255. no. 5041, pp. 200 - 203
DOI: 10.1126/science.1553547

Articles

Science, Vol 255, Issue 5041, 200-203
Copyright © 1992 by American Association for the Advancement of Science


articles

Optical analysis of synaptic vesicle recycling at the frog neuromuscular junction

WJ Betz and GS Bewick

Department of Physiology, University of Colorado School of Medicine, Denver 80262.

The fluorescent dyes FM1-43 and RH414 label motor nerve terminals in an activity-dependent fashion that involves dye uptake by synaptic vesicles that are recycling. This allows optical monitoring of vesicle recycling in living nerve terminals to determine how recycled vesicles reenter the vesicle pool. The results suggest that recycled vesicles mix with the pool morphologically and functionally. One complete cycle of release of transmitter, recycling of a vesicle, and rerelease of transmitter appears to take about 1 minute.


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Synapse Loss and Regeneration: A Mechanism for Functional Decline and Recovery after Cerebral Ischemia?.
M. J. Hasbani, S. M. Underhill, G. De Erausquin, and M. P. Goldberg (2000)
Neuroscientist 6, 110-119
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Science. ISSN 0036-8075 (print), 1095-9203 (online)