Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 15 November 1991:
Vol. 254. no. 5034, pp. 1019 - 1022
DOI: 10.1126/science.1658939
Prev | Table of Contents | Next

Articles

Science, Vol 254, Issue 5034, 1019-1022
Copyright © 1991 by American Association for the Advancement of Science

articles

Activity-dependent synaptic competition in vitro: heterosynaptic suppression of developing synapses

YJ Lo and MM Poo

Department of Biological Sciences, Columbia University, New York, NY 10027.

The development and stability of synaptic connections in the nervous system are influenced by the pattern of electrical activity and the competitive interaction between the adjacent nerve terminals. To investigate this influence, a culture system of nerve and muscle cells has been developed in which a single embryonic muscle cell is coinnervated by two spinal neurons. The effect of electrical activity on the synaptic efficacy was examined after repetitive electrical stimulation was applied to one or both neurons. Brief tetanic stimulation of one neuron resulted in immediate functional suppression of the synapse made by the unstimulated neuron innervating the same muscle cell. This heterosynaptic suppression was largely absent when the tetanic stimulation was applied concurrently to both neurons. This result demonstrates that activity-dependent synaptic competition can be studied in vitro at a cellular level.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Pro-BDNF-induced synaptic depression and retraction at developing neuromuscular synapses.
F. Yang, H.-S. Je, Y. Ji, G. Nagappan, B. Hempstead, and B. Lu (2009)
J. Cell Biol. 185, 727-741
   Abstract »    Full Text »    PDF »
GABA: A Pioneer Transmitter That Excites Immature Neurons and Generates Primitive Oscillations.
Y. Ben-Ari, J.-L. Gaiarsa, R. Tyzio, and R. Khazipov (2007)
Physiol Rev 87, 1215-1284
   Abstract »    Full Text »    PDF »
Non-genomic regulation of transmitter release by retinoic acid at developing motoneurons in Xenopus cell culture.
Y.-P. Liao, S.-Y. Ho, and J.-C. Liou (2004)
J. Cell Sci. 117, 2917-2924
   Abstract »    Full Text »    PDF »
Activity-Dependent Synaptic Competition at Mammalian Neuromuscular Junctions.
M. Buffelli, G. Busetto, C. Bidoia, M. Favero, and A. Cangiano (2004)
Physiology 19, 85-91
   Abstract »    Full Text »    PDF »
Multiple Forms of Long-Term Potentiation and Long-Term Depression Converge on a Single Interneuron in the Leech CNS.
B. D. Burrell and C. L. Sahley (2004)
J. Neurosci. 24, 4011-4019
   Abstract »    Full Text »    PDF »
Potentiation of quantal secretion by insulin-like growth factor-1 at developing motoneurons in Xenopus cell culture.
J.-C. Liou, F.-Z. Tsai, and S.-Y. Ho (2003)
J. Physiol. 553, 719-728
   Abstract »    Full Text »    PDF »
Long-Term Depression of Synaptic Inhibition Is Expressed Postsynaptically in the Developing Auditory System.
E. H. Chang, V. C. Kotak, and D. H. Sanes (2003)
J Neurophysiol 90, 1479-1488
   Abstract »    Full Text »    PDF »
Sodium Imaging of Climbing Fiber Innervation Fields in Developing Mouse Purkinje Cells.
B. Scelfo, P. Strata, and T. Knopfel (2003)
J Neurophysiol 89, 2555-2563
   Abstract »    Full Text »    PDF »
Book Review: Activity-Dependent Synaptic Plasticity: Insights from Neuromuscular Junctions.
K. E. Personius and R. J. Balice-Gordon (2002)
Neuroscientist 8, 414-422
   Abstract »    PDF »
Synaptic Precedence During Synapse Formation Between Reciprocally Connected Neurons Involves Transmitter-Receptor Interactions and AA Metabolites.
P. Lovell, B. McMahon, and N. I. Syed (2002)
J Neurophysiol 88, 1328-1338
   Abstract »    Full Text »    PDF »
Hebbian Mechanisms Revealed by Electrical Stimulation at Developing Rat Neuromuscular Junctions.
G. Busetto, M. Buffelli, E. Tognana, F. Bellico, and A. Cangiano (2000)
J. Neurosci. 20, 685-695
   Abstract »    Full Text »    PDF »
Activity-Induced Potentiation of Developing Neuromuscular Synapses.
J. Wan and M. Poo (1999)
Science 285, 1725-1728
   Abstract »    Full Text »
Long-range signaling within growing neurites mediated by neurotrophin-3.
S. Chang and S. V. Popov (1999)
PNAS 96, 4095-4100
   Abstract »    Full Text »    PDF »
Synaptic Segregation at the Developing Neuromuscular Junction.
W. Gan and J. W. Lichtman (1998)
Science 282, 1508-1511
   Abstract »    Full Text »
Enhancement of spontaneous transmitter release at neonatal mouse neuromuscular junctions by the glial cell line-derived neurotrophic factor (GDNF).
R. R Ribchester, D. Thomson, L. J Haddow, and Y. A Ushkaryov (1998)
J. Physiol. 512, 635-641
   Abstract »    Full Text »    PDF »
Differential Expression and Association of Calcium Channel alpha 1B and beta  Subunits during Rat Brain Ontogeny.
C. L. Vance, C. M. Begg, W.-L. Lee, H. Haase, T. D. Copeland, and M. W. McEnery (1998)
J. Biol. Chem. 273, 14495-14502
   Abstract »    Full Text »    PDF »
Retrograde Signaling in the Development and Modification of Synapses.
R. M. FITZSIMONDS and M.-M. POO (1998)
Physiol Rev 78, 143-170
   Abstract »    Full Text »    PDF »
Regulation of Quantal Secretion from Developing Motoneurons by Postsynaptic Activity-Dependent Release of NT-3.
J.-C. Liou and W.-M. Fu (1997)
J. Neurosci. 17, 2459-2468
   Abstract »    Full Text »    PDF »
Pathway-Specific Synaptic Plasticity: Activity-Dependent Enhancement and Suppression of Long-Term Heterosynaptic Facilitation at Converging Inputs on a Single Target.
S. Schacher, F. Wu, and Z.-Y. Sun (1997)
J. Neurosci. 17, 597-606
   Abstract »    Full Text »    PDF »
Potentiation of transmitter release by ciliary neurotrophic factor requires somatic signaling.
R Stoop and M. Poo (1995)
Science 267, 695-699
   Abstract »    PDF »
Hebbian depression of isolated neuromuscular synapses in vitro.
Y Dan and M. Poo (1992)
Science 256, 1570-1573
   Abstract »    PDF »
Expression cloning of an adenylate cyclase-coupled calcitonin receptor.
H. Lin, T. Harris, M. Flannery, A Aruffo, E. Kaji, A Gorn, L. Kolakowski Jr, H. Lodish, and Goldring SR (1991)
Science 254, 1022-1024
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)