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Science 1 November 1991:
Vol. 254. no. 5032, pp. 710 - 713
DOI: 10.1126/science.1683004
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Articles

Science, Vol 254, Issue 5032, 710-713
Copyright © 1991 by American Association for the Advancement of Science

articles

An inducible bundle-forming pilus of enteropathogenic Escherichia coli

JA Giron, AS Ho, and GK Schoolnik

Department of Microbiology and Immunology, Beckman Center, Howard Hughes Medical Institute, Stanford University, CA 94305.

Enteropathogenic Escherichia coli (EPEC), a cause of childhood diarrhea, grow on the surface of the small intestine and on cultured epithelial cells as colonies of adherent bacteria. When propagated on solid medium containing blood or attached to HEp-2 cells, EPEC express ropelike bundles of filaments, termed bundle-forming pili (BFP), that create a network of fibers that bind together the individual organisms. BFP were found to be expressed by five EPEC serogroups, each harboring a approximately 92-kilobase plasmid previously known to be important for virulence in humans. When two of these strains were cured of this plasmid, they neither expressed BFP nor grew as adherent colonies. An antiserum to BFP reduced the capacity of EPEC to infect cultured epithelial cells. BFP are composed of a repeating subunit of 19,500 daltons, the amino-terminal amino acid sequence of this subunit is homologous to that of the toxin-coregulated pilin of Vibrio cholerae.


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   Abstract »    Full Text »
Role of Tir and Intimin in the Virulence of Rabbit Enteropathogenic Escherichia coli Serotype O103:H2.
O. Marches, J.-P. Nougayrede, S. Boullier, J. Mainil, G. Charlier, I. Raymond, P. Pohl, M. Boury, J. De Rycke, A. Milon, et al. (2000)
Infect. Immun. 68, 2171-2182
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Development of a Universal Intimin Antiserum and PCR Primers.
M. Batchelor, S. Knutton, A. Caprioli, V. Huter, M. Zanial, G. Dougan, and G. Frankel (1999)
J. Clin. Microbiol. 37, 3822-3827
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Citrobacter rodentium espB Is Necessary for Signal Transduction and for Infection of Laboratory Mice.
J. V. Newman, B. A. Zabel, S. S. Jha, and D. B. Schauer (1999)
Infect. Immun. 67, 6019-6025
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Complete DNA Sequence and Structural Analysis of the Enteropathogenic Escherichia coli Adherence Factor Plasmid.
T. Tobe, T. Hayashi, C.-G. Han, G. K. Schoolnik, E. Ohtsubo, and C. Sasakawa (1999)
Infect. Immun. 67, 5455-5462
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Use of a Monoclonal Antibody against an Escherichia coli O26 Surface Protein for Detection of Enteropathogenic and Enterohemorrhagic Strains.
P. Kerr, H. Ball, B. China, J. Mainil, D. Finlay, D. Pollock, I. Wilson, and D. Mackie (1999)
Clin. Vaccine Immunol. 6, 610-614
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Role of Lactosyl Glycan Sequences in Inhibiting Enteropathogenic Escherichia coli Attachment.
R. P. Vanmaele, L. D. Heerze, and G. D. Armstrong (1999)
Infect. Immun. 67, 3302-3307
   Abstract »    Full Text »    PDF »
The meningococcal PilT protein is required for induction of intimate attachment to epithelial cells following pilus-mediated adhesion.
C. Pujol, E. Eugene, M. Marceau, and X. Nassif (1999)
PNAS 96, 4017-4022
   Abstract »    Full Text »    PDF »
Mutational Analysis of Plasmid R64 Thin Pilus Prepilin: the Entire Prepilin Sequence Is Required for Processing by Type IV Prepilin Peptidase.
T. Horiuchi and T. Komano (1998)
J. Bacteriol. 180, 4613-4620
   Abstract »    Full Text »
Type IV Pili, Transient Bacterial Aggregates, and Virulence of Enteropathogenic Escherichia coli.
D. Bieber, S. W. Ramer, C. Wu, W. J. Murray, T. Tobe, R. Fernandez, and G. K. Schoolnik (1998)
Science 280, 2114-2118
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Analysis of cis-Acting Elements Required for bfpA Expression in Enteropathogenic Escherichia coli.
V. H. Bustamante, E. Calva, and J. L. Puente (1998)
J. Bacteriol. 180, 3013-3016
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Role of Intimin and Bundle-Forming Pili in Enteropathogenic Escherichia coli Adhesion to Pediatric Intestinal Tissue In Vitro.
S. Hicks, G. Frankel, J. B. Kaper, G. Dougan, and A. D. Phillips (1998)
Infect. Immun. 66, 1570-1578
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Identification and Temperature Regulation of Legionella pneumophila Genes Involved in Type IV Pilus Biogenesis and Type II Protein Secretion.
M. R. Liles, V. K. Viswanathan, and N. P. Cianciotto (1998)
Infect. Immun. 66, 1776-1782
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A Vibrio cholerae pathogenicity island associated with epidemic and pandemic strains.
D. K. R. Karaolis, J. A. Johnson, C. C. Bailey, E. C. Boedeker, J. B. Kaper, and P. R. Reeves (1998)
PNAS 95, 3134-3139
   Abstract »    Full Text »    PDF »
Detection of Intimins alpha , beta , gamma , and delta , Four Intimin Derivatives Expressed by Attaching and Effacing Microbial Pathogens.
J. Adu-Bobie, G. Frankel, C. Bain, A. G. Goncalves, L. R. Trabulsi, G. Douce, S. Knutton, and G. Dougan (1998)
J. Clin. Microbiol. 36, 662-668
   Abstract »    Full Text »
Diffusely Adhering Escherichia coli Strains Induce Attaching and Effacing Phenotypes and Secrete Homologs of Esp Proteins.
C. Beinke, S. Laarmann, C. Wachter, H. Karch, L. Greune, and M. A. Schmidt (1998)
Infect. Immun. 66, 528-539
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Effect of Prior Experimental Human Enteropathogenic Escherichia coli Infection on Illness following Homologous and Heterologous Rechallenge.
M. S. Donnenberg, C. O. Tacket, G. Losonsky, G. Frankel, J. P. Nataro, G. Dougan, and M. M. Levine (1998)
Infect. Immun. 66, 52-58
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Diarrheagenic Escherichia coli.
J. P. Nataro and J. B. Kaper (1998)
Clin. Microbiol. Rev. 11, 142-201
   Abstract »    Full Text »    PDF »
Role of BfpF, a Member of the PilT Family of Putative Nucleotide-Binding Proteins, in Type IV Pilus Biogenesis and in Interactions between Enteropathogenic Escherichia coli and Host Cells.
R. P. Anantha, K. D. Stone, and M. S. Donnenberg (1998)
Infect. Immun. 66, 122-131
   Abstract »    Full Text »    PDF »
Activation of NF-kappa B in intestinal epithelial cells by enteropathogenic Escherichia coli.
S. D. Savkovic, A. Koutsouris, and G. Hecht (1997)
Am J Physiol Cell Physiol 273, C1160-C1167
   Abstract »    Full Text »    PDF »
Interactions Between Salmonella Typhimurium, Enteropathogenic Escherichia Coli (EPEC), and Host Epithelial Cells.
B.B. Finlay (1995)
Advances in Dental Research 9, 31-36
   Abstract »    PDF »


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