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Science 25 October 1991:
Vol. 254. no. 5031, pp. 579 - 580
DOI: 10.1126/science.1948036
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Articles

Science, Vol 254, Issue 5031, 579-580
Copyright © 1991 by American Association for the Advancement of Science

articles

Cloning of a serotonin transporter affected by antidepressants

BJ Hoffman, E Mezey, and MJ Brownstein

Laboratory of Cell Biology, National Institute of Mental Health, Bethesda, MD 20892.

A complementary DNA clone for a serotonin (5HT) transporter has been isolated from rat basophilic leukemia cells. The complementary DNA sequence predicts a 653-amino acid protein with 12 to 13 putative transmembrane domains. The 5HT transporter has significant homology to the gamma-aminobutyric acid, dopamine, and norepinephrine transporters. Uptake by CV-1 cells expressing the transporter complementary DNA resembles 5HT uptake by platelets and brain synaptosomes; it is sensitive to antidepressants, amphetamine derivatives, and cocaine.


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J. Biol. Chem. 276, 30942-30947
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Psychostimulants Differentially Regulate Serotonin Transporter Expression in Thalamocortical Neurons.
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