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Science 25 October 1991:
Vol. 254. no. 5031, pp. 578 - 579
DOI: 10.1126/science.1948035
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Articles

Science, Vol 254, Issue 5031, 578-579
Copyright © 1991 by American Association for the Advancement of Science

articles

Cloning and expression of a cocaine-sensitive rat dopamine transporter

JE Kilty, D Lorang, and SG Amara

Yale University, New Haven, CT 06510.

The action of dopamine and other monoamine neurotransmitters at synapses is terminated predominantly by high-affinity reuptake into presynaptic terminals by specific sodium-dependent neurotransmitter transport proteins. A complementary DNA encoding a rat dopamine transporter has been isolated that exhibits high sequence similarity with the previously cloned norepinephrine and gamma-aminobutyric acid transporters. Transient expression of the complementary DNA in HeLa cells confirms the cocaine sensitivity of this transporter.


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