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Science 25 October 1991:
Vol. 254. no. 5031, pp. 576 - 578
DOI: 10.1126/science.1948034
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Articles

Science, Vol 254, Issue 5031, 576-578
Copyright © 1991 by American Association for the Advancement of Science

articles

Cloning and expression of a cocaine-sensitive dopamine transporter complementary DNA

S Shimada, S Kitayama, CL Lin, A Patel, E Nanthakumar, P Gregor, M Kuhar, and G Uhl

Laboratory of Molecular Neurobiology, National Institute on Drug Abuse, Baltimore, MD 21224.

A rat dopamine (DA) transporter complementary DNA has been isolated with combined complementary DNA homology and expression approaches. The DA transporter is a 619-amino acid protein with 12 hydrophobic putative membrane-spanning domains and homology to the norepinephrine and gamma-aminobutyric acid transporters. The expressed complementary DNA confers transport of [3H]DA in Xenopus oocytes and in COS cells. Binding of the cocaine analog [3H]CFT ([3H]2 beta-carbomethoxy-3 beta-(4-fluorophenyl)tropane) to transfected COS cell membranes yields a pharmacological profile similar to that in striatal membranes.


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