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Science 25 October 1991:
Vol. 254. no. 5031, pp. 573 - 576
DOI: 10.1126/science.1948033
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Articles

Science, Vol 254, Issue 5031, 573-576
Copyright © 1991 by American Association for the Advancement of Science

articles

Quantitation of hepatic glycogenolysis and gluconeogenesis in fasting humans with 13C NMR

DL Rothman, I Magnusson, LD Katz, RG Shulman, and GI Shulman

Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510.

The rate of net hepatic glycogenolysis was assessed in humans by serially measuring hepatic glycogen concentration at 3- to 12-hour intervals during a 68-hour fast with 13C nuclear magnetic resonance spectroscopy. The net rate of gluconeogenesis was calculated by subtracting the rate of net hepatic glycogenolysis from the rate of glucose production in the whole body measured with tritiated glucose. Gluconeogenesis accounted for 64 +/- 5% (mean +/- standard error of the mean) of total glucose production during the first 22 hours of fasting. In the subsequent 14-hour and 18-hour periods of the fast, gluconeogenesis accounted for 82 +/- 5% and 96 +/- 1% of total glucose production, respectively. These data show that gluconeogenesis accounts for a substantial fraction of total glucose production even during the first 22 hours of a fast in humans.


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GLUCONEOGENESIS IS THE MAIN SOURCE OF GLUCOSE DURING FASTING.
(1991)
Journal Watch (General) 1991, 8
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Science. ISSN 0036-8075 (print), 1095-9203 (online)