Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 11 October 1991:
Vol. 254. no. 5029, pp. 270 - 274
DOI: 10.1126/science.1925581
Prev | Table of Contents | Next

Articles

Science, Vol 254, Issue 5029, 270-274
Copyright © 1991 by American Association for the Advancement of Science

articles

Structure and stability of X.G.C mismatches in the third strand of intramolecular triplexes

RF Macaya, DE Gilbert, S Malek, JS Sinsheimer, and J Feigon

Department of Chemistry and Biochemistry, University of California, Los Angeles 90024.

Intramolecular DNA triplexes that contain eight base triplets formed from the folding of a single DNA strand tolerate a single X.G.C mismatch in the third strand at acidic pH. The structure and relative stability of all four triplets that are possible involving a G.C Watson-Crick base pair were determined with one- and two-dimensional proton nuclear magnetic resonance techniques. Triplexes containing A.G.C, G.G.C, or T.G.C triplets were less stable than the corresponding parent molecule containing a C.G.C triplet. However, all mismatched bases formed specific hydrogen bonds in the major groove of the double helix. The relative effect of these mismatches on the stability of the triplex differs from the effect assayed (under different conditions) by two-dimensional gel electrophoresis and DNA cleavage with oligonucleotide EDTA.Fe(II).


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Evidence for a Triplex DNA Conformation at the bcl-2 Major Breakpoint Region of the t(14;18) Translocation.
S. C. Raghavan, P. Chastain, J. S. Lee, B. G. Hegde, S. Houston, R. Langen, C.-L. Hsieh, I. S. Haworth, and M. R. Lieber (2005)
J. Biol. Chem. 280, 22749-22760
   Abstract »    Full Text »    PDF »
An Intramolecular Triplex in the Human [IMAGE]-Globin 5`-Flanking Region Is Altered by Point Mutations Associated with Hereditary Persistence of Fetal Hemoglobin.
A. Bacolla, M. J. Ulrich, J. E. Larson, T. J. Ley, and R. D. Wells (1995)
J. Biol. Chem. 270, 24556-24563
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)