Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 11 October 1991:
Vol. 254. no. 5029, pp. 267 - 270
DOI: 10.1126/science.1833818
Prev | Table of Contents | Next

Articles

Science, Vol 254, Issue 5029, 267-270
Copyright © 1991 by American Association for the Advancement of Science

articles

The DNA binding arm of lambda repressor: critical contacts from a flexible region

ND Clarke, LJ Beamer, HR Goldberg, C Berkower, and CO Pabo

Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205.

Segments of protein that do not adopt a well-ordered conformation in the absence of DNA can still contribute to site-specific recognition of DNA. The first six residues (NH2-Ser1-Thr2-Lys3-Lys4-Lys5-Pro6-) of phage lambda repressor are flexible but are important for site-specific binding. Low-temperature x-ray crystallography and codondirected saturation mutagenesis were used to study the role of this segment. All of the functional sequences have the form [X]1-[X]2-[Lys or Arg]3-[Lys]4-[Lys or Arg]5-[X]6. A high-resolution (1.8 angstrom) crystal structure shows that Lys3 and Lys4 each make multiple hydrogen bonds with guanines and that Lys5 interacts with the phosphate backbone. The symmetry of the complex breaks down near the center of the site, and these results suggest a revision in the traditional alignment of the six lambda operator sites.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Thermodynamics of T cell receptor binding to peptide-MHC: Evidence for a general mechanism of molecular scanning.
J. J. Boniface, Z. Reich, D. S. Lyons, and M. M. Davis (1999)
PNAS 96, 11446-11451
   Abstract »    Full Text »    PDF »
The ClpXP and ClpAP proteases degrade proteins with carboxy-terminal peptide tails added by the SsrA-tagging system.
S. Gottesman, E. Roche, Y. Zhou, and R. T. Sauer (1998)
Genes & Dev. 12, 1338-1347
   Abstract »    Full Text »
Modulation of transcription factor Ets-1 DNA binding: DNA-induced unfolding of an alpha helix.
J. Petersen, J. Skalicky, L. Donaldson, L. McIntosh, T Alber, and B. Graves (1995)
Science 269, 1866-1869
   Abstract »    PDF »
Identification of Lysines within [IMAGE]1-Antichymotrypsin Important for DNA Binding.
N. Naidoo, B. S. Cooperman, Z.-m. Wang, X.-z. Liu, and H. Rubin (1995)
J. Biol. Chem. 270, 14548-14555
   Abstract »    Full Text »    PDF »
How 434 Repressor Discriminates Between O(R)1 and O(R)3.
A. C. Bell and G. B. Koudelka (1995)
J. Biol. Chem. 270, 1205-1212
   Abstract »    Full Text »    PDF »
Coupling of local folding to site-specific binding of proteins to DNA.
R. Spolar and M. Record Jr (1994)
Science 263, 777-784
   Abstract »    PDF »
Participation of non-zinc finger residues in DNA binding by two nuclear orphan receptors.
T. Wilson, R. Paulsen, K. Padgett, and J Milbrandt (1992)
Science 256, 107-110
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)