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Science 23 August 1991:
Vol. 253. no. 5022, pp. 900 - 902
DOI: 10.1126/science.1652156
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Articles

Science, Vol 253, Issue 5022, 900-902
Copyright © 1991 by American Association for the Advancement of Science

articles

Related RNA polymerase-binding regions in human RAP30/74 and Escherichia coli sigma 70

S McCracken and J Greenblatt

Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada.

RAP30/74 is a heteromeric general transcription initiation factor that binds to mammalian RNA polymerase II. The RAP30 subunit contains a region that is similar in amino acid sequence to the RNA polymerase-binding domain of the Escherichia coli transcription initiation factor sigma 70 (sigma 70). Mammalian RNA polymerase II specifically protected a serine residue in the sigma 70-related region of RAP30 from phosphorylation in vitro. In addition, human RAP30/74 bound to Escherichia coli RNA polymerase and was displaced by sigma 70. These results suggest that RAP30 and sigma 70 have functionally related RNA polymerase-binding regions.


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Molecular Genetics of the RNA Polymerase II General Transcriptional Machinery.
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The RNA Polymerase II General Transcription Factors: Past, Present, and Future.
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Identification of three regions essential for interaction between a sigma -like factor and core RNA polymerase.
P. F. Cliften, J.-Y. Park, B. P. Davis, S.-H. Jang, and J. A. Jaehning (1997)
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A Cryptic DNA Binding Domain at the COOH Terminus of TFIIIB70 Affects Formation, Stability, and Function of Preinitiation Complexes.
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RNA Polymerase II-associated Protein (RAP) 74 Binds Transcription Factor (TF) IIB and Blocks TFIIB-RAP30 Binding.
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Recycling of the general transcription factors during RNA polymerase II transcription..
L Zawel, K P Kumar, and D Reinberg (1995)
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Structure and Function of the Small Subunit of TFIIF (RAP30) from Drosophilamelanogaster.
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TFIIF-TAF-RNA polymerase II connection..
N L Henry, A M Campbell, W J Feaver, D Poon, P A Weil, and R D Kornberg (1994)
Genes & Dev. 8, 2868-2878
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Multiple functional domains of human transcription factor IIB: distinct interactions with two general transcription factors and RNA polymerase II..
I Ha, S Roberts, E Maldonado, X Sun, L U Kim, M Green, and D Reinberg (1993)
Genes & Dev. 7, 1021-1032
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The Cycling of RNA Polymerase II during Transcription.
L. Zawel, H. Lu, L.J. Cisek, J.L. Corden, and D. Reinberg (1993)
Cold Spring Harb Symp Quant Biol 58, 187-198
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Sigma factor relatives in eukaryotes.
J. Jaehning (1991)
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Direct Interaction between the Subunit RAP30 of Transcription Factor IIF (TFIIF) and RNA Polymerase Subunit 5, Which Contributes to the Association between TFIIF and RNA Polymerase II.
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A Gal4-sigma 54 Hybrid Protein That Functions as a Potent Activator of RNA Polymerase II Transcription in Yeast.
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Fluorescence-based Analyses of the Effects of Full-length Recombinant TAF130p on the Interaction of TATA Box-binding Protein with TATA Box DNA.
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