Related Content
Search Google Scholar for:
|
|
Science 12 July 1991:
Vol. 253. no. 5016, pp. 182 - 185
DOI: 10.1126/science.1853203
|
|
Articles
Science, Vol 253, Issue 5016, 182-185
Copyright © 1991 by American Association for the Advancement of Science
Novel enzymic hydrolytic dehalogenation of a chlorinated aromatic
JD Scholten,
KH Chang,
PC Babbitt,
H Charest,
M Sylvestre,
and
D Dunaway-Mariano
Department of Chemistry and Biochemistry, University of Maryland, College Park 20742.
Microbial enzyme systems may be used in the biodegradation of persistent environmental pollutants. The three polypeptide components of one such system, the 4-chlorobenzoate dehalogenase system, have been isolated, and the chemical steps of the 4-hydroxybenzoate-forming reaction that they catalyze have been identified. The genes contained within a 4.5-kilobase Pseudomonas sp. strain CBS3 chromosomal DNA fragment that encode dehalogenase activity were selectively expressed in transformed Escherichia coli. Oligonucleotide sequencing revealed a stretch of homology between the 57-kilodalton (kD) polypeptide and several magnesium adenosine triphosphate (MgATP)-cleaving enzymes that allowed MgATP and coenzyme A (CoA) to be identified as the dehalogenase cosubstrate and cofactor, respectively. The dehalogenase activity arises from two components, a 4-chlorobenzoate:CoA ligase-dehalogenase (an alpha beta dimer of the 57- and 30-kD polypeptides) and a thioesterase (the 16-kD polypeptide).
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
- Pseudomonas aeruginosa PqsA Is an Anthranilate-Coenzyme A Ligase.
- J. P. Coleman, L. L. Hudson, S. L. McKnight, J. M. Farrow III, M. W. Calfee, C. A. Lindsey, and E. C. Pesci (2008)
J. Bacteriol.
190, 1247-1255
| Abstract »
| Full Text »
| PDF »
- 4-Chlorobenzoate Uptake in Comamonas sp. Strain DJ-12 Is Mediated by a Tripartite ATP-Independent Periplasmic Transporter.
- J.-C. Chae and G. J. Zylstra (2006)
J. Bacteriol.
188, 8407-8412
| Abstract »
| Full Text »
| PDF »
- Structure, Function, and Mechanism of the Phenylacetate Pathway Hot Dog-fold Thioesterase PaaI.
- F. Song, Z. Zhuang, L. Finci, D. Dunaway-Mariano, R. Kniewel, J. A. Buglino, V. Solorzano, J. Wu, and C. D. Lima (2006)
J. Biol. Chem.
281, 11028-11038
| Abstract »
| Full Text »
| PDF »
- The BH1999 Protein of Bacillus halodurans C-125 Is Gentisyl-Coenzyme A Thioesterase.
- Z. Zhuang, F. Song, H. Takami, and D. Dunaway-Mariano (2004)
J. Bacteriol.
186, 393-399
| Abstract »
| Full Text »
| PDF »
- The Structure of 4-Hydroxybenzoyl-CoA Thioesterase from Arthrobacter sp. strain SU.
- J. B. Thoden, Z. Zhuang, D. Dunaway-Mariano, and H. M. Holden (2003)
J. Biol. Chem.
278, 43709-43716
| Abstract »
| Full Text »
| PDF »
- Characterization of the 4-Hydroxybenzoyl-Coenzyme A Thioesterase from Arthrobacter sp. Strain SU.
- Z. Zhuang, K.-H. Gartemann, R. Eichenlaub, and D. Dunaway-Mariano (2003)
Appl. Envir. Microbiol.
69, 2707-2711
| Abstract »
| Full Text »
| PDF »
- Genetics and Assembly Line Enzymology of Siderophore Biosynthesis in Bacteria.
- J. H. Crosa and C. T. Walsh (2002)
Microbiol. Mol. Biol. Rev.
66, 223-249
| Abstract »
| Full Text »
| PDF »
- Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different.
- J. L. Seffernick, M. L. de Souza, M. J. Sadowsky, and L. P. Wackett (2001)
J. Bacteriol.
183, 2405-2410
| Abstract »
| Full Text »
- Reductive, Coenzyme A-Mediated Pathway for 3-Chlorobenzoate Degradation in the Phototrophic Bacterium Rhodopseudomonas palustris.
- P. G. Egland, J. Gibson, and C. S. Harwood (2001)
Appl. Envir. Microbiol.
67, 1396-1399
| Abstract »
| Full Text »
- The Overlapping angB and angG Genes Are Encoded within the trans-Acting Factor Region of the Virulence Plasmid in Vibrio anguillarum: Essential Role in Siderophore Biosynthesis.
- T. J. Welch, S. Chai, and J. H. Crosa (2000)
J. Bacteriol.
182, 6762-6773
| Abstract »
| Full Text »
- Characterization of the angR Gene of Vibrio anguillarum: Essential Role in Virulence.
- A. M. Wertheimer, W. Verweij, Q. Chen, L. M. Crosa, M. Nagasawa, M. E. Tolmasky, L. A. Actis, and J. H. Crosa (1999)
Infect. Immun.
67, 6496-6509
| Abstract »
| Full Text »
| PDF »
- Construction and Characterization of Two Recombinant Bacteria That Grow on ortho- and para-Substituted Chlorobiphenyls.
- Y. Hrywna, T. V. Tsoi, O. V. Maltseva, J. F. Quensen III, and J. M. Tiedje (1999)
Appl. Envir. Microbiol.
65, 2163-2169
| Abstract »
| Full Text »
- Structures of Homologous Composite Transposons Carrying cbaABC Genes from Europe and North America.
- D. Di Gioia, M. Peel, F. Fava, and R. C. Wyndham (1998)
Appl. Envir. Microbiol.
64, 1940-1946
| Abstract »
| Full Text »
- From Xenobiotic to Antibiotic, Formation of Protoanemonin from 4-Chlorocatechol by Enzymes of the 3-Oxoadipate Pathway.
- R. Blasco, R.-M. Wittich, M. Mallavarapu, K. N. Timmis, and D. H. Pieper (1995)
J. Biol. Chem.
270, 29229-29235
| Abstract »
| Full Text »
| PDF »
|
|
