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Science 21 June 1991:
Vol. 252. no. 5013, pp. 1668 - 1675
DOI: 10.1126/science.2047876
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Articles

Science, Vol 252, Issue 5013, 1668-1675
Copyright © 1991 by American Association for the Advancement of Science

articles

Toward a science of metabolic engineering

JE Bailey

California Institute of Technology, Pasadena 91125.

Application of recombinant DNA methods to restructure metabolic networks can improve production of metabolite and protein products by altering pathway distributions and rates. Recruitment of heterologous proteins enables extension of existing pathways to obtain new chemical products, alter posttranslational protein processing, and degrade recalcitrant wastes. Although some of the experimental and mathematical tools required for rational metabolic engineering are available, complex cellular responses to genetic perturbations can complicate predictive design.


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