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Science 10 May 1991:
Vol. 252. no. 5007, pp. 833 - 836
DOI: 10.1126/science.1902987
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Articles

Science, Vol 252, Issue 5007, 833-836
Copyright © 1991 by American Association for the Advancement of Science

articles

Control of doublesex alternative splicing by transformer and transformer-2 in Drosophila

K Hoshijima, K Inoue, I Higuchi, H Sakamoto, and Y Shimura

Department of Biophysics, Faculty of Science, Kyoto University, Japan.

Sex-specific alternative processing of doublesex (dsx) precursor messenger RNA (pre-mRNA) regulates somatic sexual differentiation in Drosophila melanogaster. Cotransfection analyses in which the dsx gene and the female-specific transformer (tra) and transformer-2 (tra-2) complementary DNAs were expressed in Drosophila Kc cells revealed that female-specific splicing of the dsx transcript was positively regulated by the products of the tra and tra-2 genes. Furthermore, analyses of mutant constructs of dsx showed that a portion of the female-specific exon sequence was required for regulation of dsx pre-messenger RNA splicing.


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General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer..
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The role of exon sequences in splice site selection..
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