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Science 3 May 1991:
Vol. 252. no. 5006, pp. 709 - 712
DOI: 10.1126/science.1902592
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Articles

Science, Vol 252, Issue 5006, 709-712
Copyright © 1991 by American Association for the Advancement of Science

articles

In vivo footprinting of MHC class II genes: bare promoters in the bare lymphocyte syndrome

CJ Kara and LH Glimcher

Department of Cancer Biology, Harvard School of Public Health, Boston, MA 02115.

Major histocompatibility complex (MHC) class II genes are coordinately regulated and show tissue-specific expression. With the use of in vivo footprinting, common promoter sites in these genes were found to be occupied only in cells that expressed the genes, in spite of the presence of the promoter binding proteins. In vivo analysis of mutant cell lines that exhibited coordinate loss of class II MHC expression, including several from individuals with bare lymphocyte syndrome, revealed two in vivo phenotypes. One suggests a defect in gene activation, whereas the other suggests a defect in promoter accessibility.


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Discoordinate Expression of Invariant Chain and MHC Class II Genes in Class II Transactivator-Transfected Fibroblasts Defective for RFX5.
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Nuclear Factor-{kappa}B p65 Mediates the Assembly and Activation of the TNF-Responsive Element of the Murine Monocyte Chemoattractant-1 Gene.
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Interactions between the Class II Transactivator and CREB Binding Protein Increase Transcription of Major Histocompatibility Complex Class II Genes.
J. D. Fontes, S. Kanazawa, D. Jean, and B. M. Peterlin (1999)
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Cooperation among Multiple Transcription Factors Is Required for Access to Minimal T-Cell Receptor alpha -Enhancer Chromatin In Vivo.
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CIITA stimulation of transcription factor binding to major histocompatibility complex class II and associated promoters in vivo.
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Major Histocompatibility Complex Class II-associated Invariant Chain Gene Expression Is Up-regulated by Cooperative Interactions of Sp1 and NF-Y.
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Mapping of the Interaction Site of the Defective Transcription Factor in the Class II Major Histocompatibility Complex Mutant Cell Line Clone-13 to the Divergent X2-Box.
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