Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 19 April 1991:
Vol. 252. no. 5004, pp. 446 - 448
DOI: 10.1126/science.2017684
Prev | Table of Contents | Next

Articles

Science, Vol 252, Issue 5004, 446-448
Copyright © 1991 by American Association for the Advancement of Science

articles

Primary structure and functional activity of a phosphatidylinositol-glycan-specific phospholipase D

BJ Scallon, WJ Fung, TC Tsang, S Li, H Kado-Fong, KS Huang, and JP Kochan

Department of Molecular/Cellular Biology and Biochemistry, Hoffmann-La Roche, Inc., Nutley, NJ 07110.

A phosphatidylinositol-glycan-specific phospholipase D (PI-G PLD) that specifically hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol-glycans (PI-Gs) has recently been purified from human and bovine sera. The primary structure of bovine PI-G PLD has now been determined and the functional activity of the enzyme has been studied. Expression of PI-G PLD complementary DNA in COS cells produced a protein that specifically hydrolyzed the inositol phosphate linkage of the PI-G anchor. Cotransfection of PI-G PLD with a PI-G-anchored protein resulted in the secretion of the PI-G-anchored protein. The results suggest that the expression of PI-G PLD may influence the expression and location of PI-G-anchored proteins.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
PGAP2 Is Essential for Correct Processing and Stable Expression of GPI-anchored Proteins.
Y. Tashima, R. Taguchi, C. Murata, H. Ashida, T. Kinoshita, and Y. Maeda (2006)
Mol. Biol. Cell 17, 1410-1420
   Abstract »    Full Text »    PDF »
Monozygotic Twin Model Reveals Novel Embryo-Induced Transcriptome Changes of Bovine Endometrium in the Preattachment Period.
C. Klein, S. Bauersachs, S. E. Ulbrich, R. Einspanier, H. H.D. Meyer, S. E.M. Schmidt, H.-D. Reichenbach, M. Vermehren, F. Sinowatz, H. Blum, et al. (2006)
Biol Reprod 74, 253-264
   Abstract »    Full Text »    PDF »
Molecular Characterization of a Phospholipase D Generating Anandamide and Its Congeners.
Y. Okamoto, J. Morishita, K. Tsuboi, T. Tonai, and N. Ueda (2004)
J. Biol. Chem. 279, 5298-5305
   Abstract »    Full Text »    PDF »
An Iron-dependent Bacterial Phospholipase D Reminiscent of Purple Acid Phosphatases.
C. Zambonelli and M. F. Roberts (2003)
J. Biol. Chem. 278, 13706-13711
   Abstract »    Full Text »    PDF »
Increased expression of GPI-specific phospholipase D in mouse models of type 1 diabetes.
M. A. Deeg, R. F. Bowen, M. D. Williams, L. K. Olson, E. A. Kirk, and R. C. LeBoeuf (2001)
Am J Physiol Endocrinol Metab 281, E147-E154
   Abstract »    Full Text »    PDF »
GPI-specific phospholipase D associates with an apoA-I- and apoA-IV-containing complex.
M. A. Deeg, E. L. Bierman, and M. C. Cheung (2001)
J. Lipid Res. 42, 442-451
   Abstract »    Full Text »
Structure of the glycosylphosphatidylinositol anchor of an arabinogalactan protein from Pyrus communis suspension-cultured cells.
D. Oxley and A. Bacic (1999)
PNAS 96, 14246-14251
   Abstract »    Full Text »    PDF »
Glycosylphosphatidylinositol-Specific Phospholipase D Is Expressed by Macrophages in Human Atherosclerosis and Colocalizes With Oxidation Epitopes.
K. D. O'Brien, C. Pineda, W. S. Chiu, R. Bowen, and M. A. Deeg (1999)
Circulation 99, 2876-2882
   Abstract »    Full Text »    PDF »
Regulation of Glycosylphosphatidylinositol-Specific Phospholipase D Secretion from {beta}TC3 cells.
M. A. Deeg and C. B. Verchere (1997)
Endocrinology 138, 819-826
   Abstract »    Full Text »    PDF »
Folding of the N-terminal, ligand-binding region of integrin alpha -subunits into a beta -propeller domain.
T. A. Springer (1997)
PNAS 94, 65-72
   Abstract »    Full Text »    PDF »
Phosphatidylinositol Phospholipase C Is Activated Allosterically by the Aminoglycoside G418. 2-DEOXY-2-FLUORO-SCYLLO-INOSITOL-1-O-DODECYLPHOSPHONATE AND ITS ANALOGS INHIBIT GLYCOSYLPHOSPHATIDYLINOSITOL PHOSPHOLIPASE C.
J. C. Morris, L. Ping-Sheng, H.-X. Zhai, T.-Y. Shen, and K. Mensa-Wilmot (1996)
J. Biol. Chem. 271, 15468-15477
   Abstract »    Full Text »    PDF »
Cell surface expression of a functional rubella virus E1 glycoprotein by addition of a GPI anchor.
E Bernasconi, N Fasel, and R Wittek (1996)
J. Cell Sci. 109, 1195-1201
   Abstract »    PDF »
Human ADP-ribosylation Factor-activated Phosphatidylcholine-specific Phospholipase D Defines a New and Highly Conserved Gene Family.
S. M. Hammond, Y. M. Altshuller, T.-C. Sung, S. A. Rudge, K. Rose, J. Engebrecht, A. J. Morris, and M. A. Frohman (1995)
J. Biol. Chem. 270, 29640-29643
   Abstract »    Full Text »    PDF »
Identification and Characterization of a Novel Protein (p137) Which Transcytoses Bidirectionally in Caco-2 Cells.
J. A. Ellis and J. P. Luzio (1995)
J. Biol. Chem. 270, 20717-20723
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)