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Science 22 March 1991:
Vol. 251. no. 5000, pp. 1490 - 1493
DOI: 10.1126/science.2006423
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Articles

Science, Vol 251, Issue 5000, 1490-1493
Copyright © 1991 by American Association for the Advancement of Science

articles

Isolation of a rel-related human cDNA that potentially encodes the 65-kD subunit of NF-kappa B

SM Ruben, PJ Dillon, R Schreck, T Henkel, CH Chen, M Maher, PA Baeuerle, and CA Rosen

Department of Molecular Oncology and Virology, Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110-1199.

A DNA probe that spanned a domain conserved among the proto-oncogene c-rel, the Drosophila morphogen dorsal, and the p50 DNA binding subunit of NF-kappa B was generated from Jurkat T cell complementary DNA with the polymerase chain reaction (PCR) and degenerate oligonucleotides. This probe was used to identify a rel-related complementary DNA that hybridized to a 2.6-kilobase messenger RNA present in human T and B lymphocytes. In vitro transcription and translation of the complementary DNA resulted in the synthesis of a protein with an apparent molecular size of 65 kilodaltons (kD). The translated protein showed weak DNA binding with a specificity for the kappa B binding motif. This protein-DNA complex comigrated with the complex obtained with the purified human p65 NF-kappa B subunit and binding was inhibited by I kappa B-alpha and -beta proteins. In addition, the 65-kD protein associated with the p50 subunit of NF-kappa B and the kappa B probe to form a complex with the same electrophoretic mobility as the NF-kappa B-DNA complex. Therefore the rel-related 65-kD protein may represent the p65 subunit of the active NF-kappa B transcription factor complex.


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NF-kappa B subunit regulation in nontransformed CD4+ T lymphocytes.
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Science 256, 1452-1456
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I-Rel: a novel rel-related protein that inhibits NF-kappa B transcriptional activity..
S M Ruben, J F Klement, T A Coleman, M Maher, C H Chen, and C A Rosen (1992)
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Transcriptional regulation of the HIV-1 promoter by NF-kappa B in vitro..
M Kretzschmar, M Meisterernst, C Scheidereit, G Li, and R G Roeder (1992)
Genes & Dev. 6, 761-774
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Independent modes of transcriptional activation by the p50 and p65 subunits of NF-kappa B..
T Fujita, G P Nolan, S Ghosh, and D Baltimore (1992)
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Identification of a Naturally Occurring Transforming Variant of the p65 Subunit of NF-KB.
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Genes & Dev. 6, 619-630
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Rel-associated pp40: an inhibitor of the rel family of transcription factors.
N Davis, S Ghosh, D. Simmons, P Tempst, H. Liou, D Baltimore, and H. Bose Jr (1991)
Science 253, 1268-1271
   Abstract »    PDF »
The rel-associated pp40 protein prevents DNA binding of Rel and NF-kappa B: relationship with I kappa B beta and regulation by phosphorylation..
L D Kerr, J Inoue, N Davis, E Link, P A Baeuerle, H R Bose, and I M Verma (1991)
Genes & Dev. 5, 1464-1476
   Abstract »    PDF »
Dynamics of NF kappa B and Ikappa Balpha Studied with Green Fluorescent Protein (GFP) Fusion Proteins. INVESTIGATION OF GFP-p65 BINDING TO DNA BY FLUORESCENCE RESONANCE ENERGY TRANSFER.
J. A. Schmid, A. Birbach, R. Hofer-Warbinek, M. Pengg, U. Burner, P. G. Furtmuller, B. R. Binder, and R. de Martin (2000)
J. Biol. Chem. 275, 17035-17042
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