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Science 8 March 1991:
Vol. 251. no. 4998, pp. 1233 - 1236
DOI: 10.1126/science.1848726
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Articles

Science, Vol 251, Issue 4998, 1233-1236
Copyright © 1991 by American Association for the Advancement of Science

articles

Enhanced motility in NIH 3T3 fibroblasts that overexpress gelsolin

CC Cunningham, TP Stossel, and DJ Kwiatkowski

Hematology-Oncology Unit, Massachusetts General Hospital, Boston 02129.

Increasing the content of the actin-binding protein gelsolin in cultured mouse fibroblasts by up to 125 percent by gene transfection proportionally enhanced the rate at which the cells migrated through porous filters toward a gradient of serum and closed a wound made on a confluent monolayer of cells in a tissue culture dish. These results provide direct evidence that gelsolin, which promotes both actin assembly and disassembly in vitro, is an important element in fibroblast locomotion and demonstrate that the manipulation of intracellular machinery can increase cell motility.


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