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Science 8 March 1991:
Vol. 251. no. 4998, pp. 1228 - 1231
DOI: 10.1126/science.1900952
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Articles

Science, Vol 251, Issue 4998, 1228-1231
Copyright © 1991 by American Association for the Advancement of Science

articles

Split anergy in a CD8+ T cell: receptor-dependent cytolysis in the absence of interleukin-2 production

GR Otten and RN Germain

Lymphocyte Biology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.

Engagement of the antigen-specific receptor (TCR) of CD4+ T lymphocytes without a second (costimulatory) signal prevents the subsequent production of interleukin-2 (IL-2) by these cells. Because IL-2 is a key immunoregulatory lymphokine and is also produced by a subset of CD8+ T cells that are able to kill target cells, the effect of engaging the TCR of one such clone in the absence of costimulatory signals was examined. The capacity for TCR-dependent IL-2 production was lost, indicating comparable costimulator-dependent signaling requirements for IL-2 production in CD4+ and CD8+ T cells. However, TCR-mediated cytotoxicity was not impaired, implying that costimulation is required for only certain TCR-dependent effector functions.


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