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Science 15 February 1991:
Vol. 251. no. 4995, pp. 767 - 773
DOI: 10.1126/science.1990438
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Articles

Science, Vol 251, Issue 4995, 767-773
Copyright © 1991 by American Association for the Advancement of Science

articles

Light-directed, spatially addressable parallel chemical synthesis

SP Fodor, JL Read, MC Pirrung, L Stryer, AT Lu, and D Solas

Affymax Research Institute, Palo Alto, CA 94304.

Solid-phase chemistry, photolabile protecting groups, and photolithography have been combined to achieve light-directed, spatially addressable parallel chemical synthesis to yield a highly diverse set of chemical products. Binary masking, one of many possible combinatorial synthesis strategies, yields 2n compounds in n chemical steps. An array of 1024 peptides was synthesized in ten steps, and its interaction with a monoclonal antibody was assayed by epifluorescence microscopy. High-density arrays formed by light-directed synthesis are potentially rich sources of chemical diversity for discovering new ligands that bind to biological receptors and for elucidating principles governing molecular interactions. The generality of this approach is illustrated by the light-directed synthesis of a dinucleotide. Spatially directed synthesis of complex compounds could also be used for microfabrication of devices.


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Complex Transcriptional Responses to Macromolecular Damaging Agents: Regulatory Responses Specific for SN2 Alkylation and the MAG1 Gene.
T.J. BEGLEY, S.A. JELINSKY, and L.D. SAMSON (2000)
Cold Spring Harb Symp Quant Biol 65, 383-394
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Science. ISSN 0036-8075 (print), 1095-9203 (online)