Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 4 January 1991:
Vol. 251. no. 4989, pp. 81 - 85
DOI: 10.1126/science.1898782
Prev | Table of Contents | Next

Articles

Science, Vol 251, Issue 4989, 81-85
Copyright © 1991 by American Association for the Advancement of Science

articles

Whole animal cell sorting of Drosophila embryos

MA Krasnow, S Cumberledge, G Manning, LA Herzenberg, and GP Nolan

Department of Biochemistry, Stanford University, CA 94305.

Use of primary culture cells has been limited by the inability to purify most types of cells, particularly cells from early developmental stages. In whole animal cell sorting (WACS), live cells derived from animals harboring a lacZ transgene are purified according to their level of beta-galactosidase expression with a fluorogenic beta-galactosidase substrate and fluorescence-activated cell sorting. With WACS, incipient posterior compartment cells that express the engrailed gene were purified from early Drosophila embryos. Neuronal precursor cells were also purified, and they differentiated into neurons with high efficiency in culture. Because there are many lacZ strains, it may be possible to purify most types of Drosophila cells. The same approach is also applicable to other organisms for which germ-line transformation is possible.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Cell survival and proliferation are modified by insulin-like growth factor 2 between days 9 and 10 of mouse gestation.
J. L. Burns and A. B. Hassan (2001)
Development 128, 3819-3830
   Abstract »    Full Text »    PDF »
Characterization of differentially expressed genes in purified Drosophila follicle cells: Toward a general strategy for cell type-specific developmental analysis.
Z. Bryant, L. Subrahmanyan, M. Tworoger, L. LaTray, C.-R. Liu, M.-J. Li, G. van den Engh, and H. Ruohola-Baker (1999)
PNAS 96, 5559-5564
   Abstract »    Full Text »    PDF »
Detection and Isolation of Gene-Corrected Cells in Gaucher Disease Via a Fluorescence-Activated Cell Sorter Assay for Lysosomal Glucocerebrosidase Activity.
M. Lorincz, L. A. Herzenberg, Z. Diwu, J. A. Barranger, and W. G. Kerr (1997)
Blood 89, 3412-3420
   Abstract »    Full Text »    PDF »
Expression of the Ly-6E.1 (Sca-1) transgene in adult hematopoietic stem cells and the developing mouse embryo.
C Miles, M. Sanchez, A Sinclair, and E Dzierzak (1997)
Development 124, 537-547
   Abstract »    PDF »
The DNA binding domain of retinoic acid receptor beta is required for ligand-dependent suppression of proliferation. Application of general purpose mammalian coexpression vectors.
J. Frangioni, N Moghal, A Stuart-Tilley, B. Neel, and S. Alper (1994)
J. Cell Sci. 107, 827-838
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)