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Science 14 December 1990:
Vol. 250. no. 4987, pp. 1570 - 1573
DOI: 10.1126/science.2125748
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Articles

Science, Vol 250, Issue 4987, 1570-1573
Copyright © 1990 by American Association for the Advancement of Science

articles

An ancient group I intron shared by eubacteria and chloroplasts

MG Kuhsel, R Strickland, and JD Palmer

Department of Biology, Indiana University, Bloomington 47405.

Introns have been found in the genomes of all major groups of organisms except eubacteria. The presence of introns in chloroplasts and mitochondria, both of which are of eubacterial origin, has been interpreted as evidence either for the recent acquisition of introns by organelles or for the loss of introns from their eubacterial progenitors. The gene for the leucine transfer RNA with a UAA anticodon [tRNALeu (UAA)] from five diverse cyanobacteria and several major groups of chloroplasts contains a single group I intron. The intron is conserved in secondary structure and primary sequence, and occupies the same position, within the UAA anticodon. The homology of the intron across chloroplasts and cyanobacteria implies that it was present in their common ancestor and that it has been maintained in their genomes for at least 1 billion years.


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M. Turmel, C. Otis, and C. Lemieux (1999)
PNAS 96, 10248-10253
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Unexpected abundance of self-splicing introns in the genome of bacteriophage Twort: Introns in multiple genes, a single gene with three introns, and exon skipping by group I ribozymes.
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Complex Evolutionary Patterns of tRNAUAALeu Group I Introns in Cyanobacterial Radiation.
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tRNA Splicing.
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Sequence Variation of the tRNALeu Intron as a Marker for Genetic Diversity and Specificity of Symbiotic Cyanobacteria in Some Lichens.
P. Paulsrud and P. Lindblad (1998)
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M Belfort (1993)
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Activation of the catalytic core of a group I intron by a remote 3' splice junction..
F Michel, L Jaeger, E Westhof, R Kuras, F Tihy, M Q Xu, and D A Shub (1992)
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Introns pop up in new places--what does it mean?.
M Barinaga (1990)
Science 250, 1512
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Bacterial origin of a chloroplast intron: conserved self-splicing group I introns in cyanobacteria.
M. Xu, S. Kathe, H Goodrich-Blair, S. Nierzwicki-Bauer, and D. Shub (1990)
Science 250, 1566-1570
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