Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 9 November 1990:
Vol. 250. no. 4982, pp. 827 - 830
DOI: 10.1126/science.2237434
Prev | Table of Contents | Next

Articles

Science, Vol 250, Issue 4982, 827-830
Copyright © 1990 by American Association for the Advancement of Science

articles

Inhibition of the complement cascade by the major secretory protein of vaccinia virus

GJ Kotwal, SN Isaacs, R McKenzie, MM Frank, and B Moss

Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.

The complement system contributes to host defenses against invasion by infectious agents. A 35-kilodalton protein, encoded by vaccinia virus and secreted from infected cells, has sequence similarities to members of a gene family that includes complement control proteins. Biochemical and genetic studies showed that the viral protein binds to derivatives of the fourth component of complement and inhibits the classical complement cascade, suggesting that it serves as a defense molecule to help the virus evade the consequences of complement activation.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Heavily Isotype-Dependent Protective Activities of Human Antibodies against Vaccinia Virus Extracellular Virion Antigen B5.
M. R.-E.-I. Benhnia, M. M. McCausland, J. Laudenslager, S. W. Granger, S. Rickert, L. Koriazova, T. Tahara, R. T. Kubo, S. Kato, and S. Crotty (2009)
J. Virol. 83, 12355-12367
   Abstract »    Full Text »    PDF »
Smallpox vaccines induce antibodies to the immunomodulatory, secreted vaccinia virus complement control protein.
J. E. Adamo, C. A. Meseda, J. P. Weir, and M. J. Merchlinsky (2009)
J. Gen. Virol. 90, 2604-2608
   Abstract »    Full Text »    PDF »
Smallpox Inhibitor of Complement Enzymes (SPICE): Dissecting Functional Sites and Abrogating Activity.
M. K. Liszewski, M. K. Leung, R. Hauhart, C. J. Fang, P. Bertram, and J. P. Atkinson (2009)
J. Immunol. 183, 3150-3159
   Abstract »    Full Text »    PDF »
Acidic residues in the membrane-proximal stalk region of vaccinia virus protein B5 are required for glycosaminoglycan-mediated disruption of the extracellular enveloped virus outer membrane.
K. L. Roberts, A. Breiman, G. C. Carter, H. A. Ewles, M. Hollinshead, M. Law, and G. L. Smith (2009)
J. Gen. Virol. 90, 1582-1591
   Abstract »    Full Text »    PDF »
Inhibition of Complement and CD14 Attenuates the Escherichia coli-Induced Inflammatory Response in Porcine Whole Blood.
E. B. Thorgersen, A. Pharo, K. Haverson, A. K. Axelsen, P. Gaustad, G. J. Kotwal, G. Sfyroera, and T. E. Mollnes (2009)
Infect. Immun. 77, 725-732
   Abstract »    Full Text »    PDF »
Vaccinia Virus Extracellular Enveloped Virion Neutralization In Vitro and Protection In Vivo Depend on Complement.
M. R.-E.-I. Benhnia, M. M. McCausland, J. Moyron, J. Laudenslager, S. Granger, S. Rickert, L. Koriazova, R. Kubo, S. Kato, and S. Crotty (2009)
J. Virol. 83, 1201-1215
   Abstract »    Full Text »    PDF »
Smallpox Inhibitor of Complement Enzymes (SPICE): Regulation of Complement Activation on Cells and Mechanism of Its Cellular Attachment.
M. K. Liszewski, P. Bertram, M. K. Leung, R. Hauhart, L. Zhang, and J. P. Atkinson (2008)
J. Immunol. 181, 4199-4207
   Abstract »    Full Text »    PDF »
Cell Surface Expression of the Vaccinia Virus Complement Control Protein Is Mediated by Interaction with the Viral A56 Protein and Protects Infected Cells from Complement Attack.
N. M. Girgis, B. C. DeHaven, X. Fan, K. M. Viner, M. Shamim, and S. N. Isaacs (2008)
J. Virol. 82, 4205-4214
   Abstract »    Full Text »    PDF »
Complement Is an Essential Component of the Immune Response to Adeno-Associated Virus Vectors.
A. K. Zaiss, M. J. Cotter, L. R. White, S. A. Clark, N. C. W. Wong, V. M. Holers, J. S. Bartlett, and D. A. Muruve (2008)
J. Virol. 82, 2727-2740
   Abstract »    Full Text »    PDF »
Distinct Gene Expression Profiling after Infection of Immature Human Monocyte-Derived Dendritic Cells by the Attenuated Poxvirus Vectors MVA and NYVAC.
S. Guerra, J. L. Najera, J. M. Gonzalez, L. A. Lopez-Fernandez, N. Climent, J. M. Gatell, T. Gallart, and M. Esteban (2007)
J. Virol. 81, 8707-8721
   Abstract »    Full Text »    PDF »
Association of Vaccinia Virus Fusion Regulatory Proteins with the Multicomponent Entry/Fusion Complex.
T. R. Wagenaar and B. Moss (2007)
J. Virol. 81, 6286-6293
   Abstract »    Full Text »    PDF »
Structure and Regulatory Profile of the Monkeypox Inhibitor of Complement: Comparison to Homologs in Vaccinia and Variola and Evidence for Dimer Formation.
M. K. Liszewski, M. K. Leung, R. Hauhart, R. M. L. Buller, P. Bertram, X. Wang, A. M. Rosengard, G. J. Kotwal, and J. P. Atkinson (2006)
J. Immunol. 176, 3725-3734
   Abstract »    Full Text »    PDF »
Identification of Complement Regulatory Domains in Vaccinia Virus Complement Control Protein.
J. Mullick, J. Bernet, Y. Panse, S. Hallihosur, A. K. Singh, and A. Sahu (2005)
J. Virol. 79, 12382-12393
   Abstract »    Full Text »    PDF »
Synergy between Two Active Sites of Human Complement Receptor Type 1 (CD35) in Complement Regulation: Implications for the Structure of the Classical Pathway C3 Convertase and Generation of More Potent Inhibitors.
M. Krych-Goldberg, R. E. Hauhart, T. Porzukowiak, and J. P. Atkinson (2005)
J. Immunol. 175, 4528-4535
   Abstract »    Full Text »    PDF »
Vaccinia Virus Induces Strong Immunoregulatory Cytokine Production in Healthy Human Epidermal Keratinocytes: a Novel Strategy for Immune Evasion.
L. Liu, Z. Xu, R. C. Fuhlbrigge, V. Pena-Cruz, J. Lieberman, and T. S. Kupper (2005)
J. Virol. 79, 7363-7370
   Abstract »    Full Text »    PDF »
Electrostatic Modeling Predicts the Activities of Orthopoxvirus Complement Control Proteins.
G. Sfyroera, M. Katragadda, D. Morikis, S. N. Isaacs, and J. D. Lambris (2005)
J. Immunol. 174, 2143-2151
   Abstract »    Full Text »    PDF »
The Kaposi's Sarcoma-associated Herpesvirus Complement Control Protein Mimics Human Molecular Mechanisms for Inhibition of the Complement System.
L. Mark, W. H. Lee, O. B. Spiller, D. Proctor, D. J. Blackbourn, B. O. Villoutreix, and A. M. Blom (2004)
J. Biol. Chem. 279, 45093-45101
   Abstract »    Full Text »    PDF »
Secreted Immunomodulatory Viral Proteins as Novel Biotherapeutics.
A. Lucas and G. McFadden (2004)
J. Immunol. 173, 4765-4774
   Abstract »    Full Text »    PDF »
Kinetic Analysis of the Interactions between Vaccinia Virus Complement Control Protein and Human Complement Proteins C3b and C4b.
J. Bernet, J. Mullick, Y. Panse, P. B. Parab, and A. Sahu (2004)
J. Virol. 78, 9446-9457
   Abstract »    Full Text »    PDF »
Structure of vaccinia complement protein in complex with heparin and potential implications for complement regulation.
V. K. Ganesh, S. A. Smith, G. J. Kotwal, and K. H. M. Murthy (2004)
PNAS 101, 8924-8929
   Abstract »    Full Text »    PDF »
Restoration of Complement-Enhanced Neutralization of Vaccinia Virus Virions by Novel Monoclonal Antibodies Raised against the Vaccinia Virus Complement Control Protein.
S. N. Isaacs, E. Argyropoulos, G. Sfyroera, S. Mohammad, and J. D. Lambris (2003)
J. Virol. 77, 8256-8262
   Abstract »    Full Text »    PDF »
Poxvirus Immunomodulatory Strategies: Current Perspectives.
J. B. Johnston and G. McFadden (2003)
J. Virol. 77, 6093-6100
   Full Text »    PDF »
Kaposi's Sarcoma-Associated Herpesvirus (Human Herpesvirus 8) Open Reading Frame 4 Protein (Kaposica) Is a Functional Homolog of Complement Control Proteins.
J. Mullick, J. Bernet, A. K. Singh, J. D. Lambris, and A. Sahu (2003)
J. Virol. 77, 3878-3881
   Abstract »    Full Text »    PDF »
Functional Activity of the Complement Regulator Encoded by Kaposi's Sarcoma-associated Herpesvirus.
O. B. Spiller, D. J. Blackbourn, L. Mark, D. G. Proctor, and A. M. Blom (2003)
J. Biol. Chem. 278, 9283-9289
   Abstract »    Full Text »    PDF »
Virus complement evasion strategies.
H. W. Favoreel, G. R. Van de Walle, H. J. Nauwynck, and M. B. Pensaert (2003)
J. Gen. Virol. 84, 1-15
   Abstract »    Full Text »    PDF »
The formation and function of extracellular enveloped vaccinia virus.
G. L. Smith, A. Vanderplasschen, and M. Law (2002)
J. Gen. Virol. 83, 2915-2931
   Abstract »    Full Text »    PDF »
The vaccinia virus C12L protein inhibits mouse IL-18 and promotes virus virulence in the murine intranasal model.
J. A. Symons, E. Adams, D. C. Tscharke, P. C. Reading, H. Waldmann, and G. L. Smith (2002)
J. Gen. Virol. 83, 2833-2844
   Abstract »    Full Text »    PDF »
From the Cover: Variola virus immune evasion design: Expression of a highly efficient inhibitor of human complement.
A. M. Rosengard, Y. Liu, Z. Nie, and R. Jimenez (2002)
PNAS 99, 8808-8813
   Abstract »    Full Text »    PDF »
The sequence of camelpox virus shows it is most closely related to variola virus, the cause of smallpox.
C. Gubser and G. L. Smith (2002)
J. Gen. Virol. 83, 855-872
   Abstract »    Full Text »    PDF »
The vaccinia virus soluble interferon-{gamma} receptor is a homodimer.
A. Alcami and G. L. Smith (2002)
J. Gen. Virol. 83, 545-549
   Abstract »    Full Text »    PDF »
Complement Evasion by Borrelia burgdorferi: Serum-Resistant Strains Promote C3b Inactivation.
A. Alitalo, T. Meri, L. Ramo, T. S. Jokiranta, T. Heikkila, I. J. T. Seppala, J. Oksi, M. Viljanen, and S. Meri (2001)
Infect. Immun. 69, 3685-3691
   Abstract »    Full Text »    PDF »
Complement- First of Two Parts.
M. J. Walport (2001)
N. Engl. J. Med. 344, 1058-1066
   Full Text »    PDF »
A Functional NSP4 Enterotoxin Peptide Secreted from Rotavirus-Infected Cells.
M. Zhang, C. Q.-Y. Zeng, A. P. Morris, and M. K. Estes (2000)
J. Virol. 74, 11663-11670
   Abstract »    Full Text »
Novel Mechanism of Antibody-Independent Complement Neutralization of Herpes Simplex Virus Type 1.
H. M. Friedman, L. Wang, M. K. Pangburn, J. D. Lambris, and J. Lubinski (2000)
J. Immunol. 165, 4528-4536
   Abstract »    Full Text »    PDF »
Conserved Surface-Exposed K/R-X-K/R Motifs and Net Positive Charge on Poxvirus Complement Control Proteins Serve as Putative Heparin Binding Sites and Contribute to Inhibition of Molecular Interactions with Human Endothelial Cells: a Novel Mechanism for Evasion of Host Defense.
S. A. Smith, N. P. Mullin, J. Parkinson, S. N. Shchelkunov, A. V. Totmenin, V. N. Loparev, R. Srisatjaluk, D. N. Reynolds, K. L. Keeling, D. E. Justus, et al. (2000)
J. Virol. 74, 5659-5666
   Abstract »    Full Text »
Humoral response to herpes simplex virus is complement-dependent.
X. J. Da Costa, M. A. Brockman, E. Alicot, M. Ma, M. B. Fischer, X. Zhou, D. M. Knipe, and M. C. Carroll (1999)
PNAS 96, 12708-12712
   Abstract »    Full Text »    PDF »
IL-18 binding and inhibition of interferon gamma induction by human poxvirus-encoded proteins.
Y. Xiang and B. Moss (1999)
PNAS 96, 11537-11542
   Abstract »    Full Text »    PDF »
Murine Gammaherpesvirus 68 Encodes a Functional Regulator of Complement Activation.
S. B. Kapadia, H. Molina, V. van Berkel, S. H. Speck, and H. W. Virgin IV (1999)
J. Virol. 73, 7658-7670
   Abstract »    Full Text »
Interaction of Vaccinia Virus Complement Control Protein with Human Complement Proteins: Factor I-Mediated Degradation of C3b to iC3b1 Inactivates the Alternative Complement Pathway.
A. Sahu, S. N. Isaacs, A. M. Soulika, and J. D. Lambris (1998)
J. Immunol. 160, 5596-5604
   Abstract »    Full Text »    PDF »
Activation of Caspases in Pig Kidney Cells Infected with Wild-Type and CrmA/SPI-2 Mutants of Cowpox and Rabbitpox Viruses.
J. Macen, A. Takahashi, K. B. Moon, R. Nathaniel, P. C. Turner, and R. W. Moyer (1998)
J. Virol. 72, 3524-3533
   Abstract »    Full Text »    PDF »
A Novel Sialic Acid Binding Site on Factor H Mediates Serum Resistance of Sialylated Neisseria gonorrhoeae.
S. Ram, A. K. Sharma, S. D. Simpson, S. Gulati, D. P. McQuillen, M. K. Pangburn, and P. A. Rice (1998)
J. Exp. Med. 187, 743-752
   Abstract »    Full Text »    PDF »
Functional Analysis of Vaccinia Virus B5R Protein: Essential Role in Virus Envelopment Is Independent of a Large Portion of the Extracellular Domain.
E. Herrera, M. del Mar Lorenzo, R. Blasco, and S. N. Isaacs (1998)
J. Virol. 72, 294-302
   Abstract »    Full Text »    PDF »
Why the smallpox virus stocks should not be destroyed.
W. Joklik, B Moss, B. Fields, D. Bishop, and L. Sandakhchiev (1993)
Science 262, 1225-1226
   PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)