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Science 9 November 1990:
Vol. 250. no. 4982, pp. 786 - 791
DOI: 10.1126/science.2173140
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Articles

Science, Vol 250, Issue 4982, 786-791
Copyright © 1990 by American Association for the Advancement of Science

articles

Cell cycle control of DNA replication by a homologue from human cells of the p34cdc2 protein kinase

G D'Urso, RL Marraccino, DR Marshak, and JM Roberts

Department of Basic Sciences, Fred Hutchinson Cancer Center, Seattle, WA 98104.

The regulation of DNA replication during the eukaryotic cell cycle was studied in a system where cell free replication of simian virus 40 (SV40) DNA was used as a model for chromosome replication. A factor, RF-S, was partially purified from human S phase cells based on its ability to activate DNA replication in extracts from G1 cells. RF-S contained a human homologue of the Schizosaccharomyces pombe p34cdc2 kinase, and this kinase was necessary for RF-S activity. The limiting step in activation of the p34 kinase at the G1 to S transition may be its association with a cyclin since addition of cyclin A to a G1 extract was sufficient to start DNA replication. These observations suggest that the role of p34cdc2 in controlling the start of DNA synthesis has been conserved in evolution.


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