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Science 14 September 1990:
Vol. 249. no. 4974, pp. 1303 - 1306
DOI: 10.1126/science.1975955
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Articles

Science, Vol 249, Issue 4974, 1303-1306
Copyright © 1990 by American Association for the Advancement of Science

articles

Cloning and expression of a rat brain GABA transporter

J Guastella, N Nelson, H Nelson, L Czyzyk, S Keynan, MC Miedel, N Davidson, HA Lester, and BI Kanner

Division of Biology, California Institute of Technology, Pasadena 91125.

A complementary DNA clone (designated GAT-1) encoding a transporter for the neurotransmitter gamma-aminobutyric acid (GABA) has been isolated from rat brain, and its functional properties have been examined in Xenopus oocytes. Oocytes injected with GAT-1 synthetic messenger RNA accumulated [3H]GABA to levels above control values. The transporter encoded by GAT-1 has a high affinity for GABA, is sodium-and chloride-dependent, and is pharmacologically similar to neuronal GABA transporters. The GAT-1 protein shares antigenic determinants with a native rat brain GABA transporter. The nucleotide sequence of GAT-1 predicts a protein of 599 amino acids with a molecular weight of 67 kilodaltons. Hydropathy analysis of the deduced protein suggests multiple transmembrane regions, a feature shared by several cloned transporters; however, database searches indicate that GAT-1 is not homologous to any previously identified proteins. Therefore, GAT-1 appears to be a member of a previously uncharacterized family of transport molecules.


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E. R. Bennett and B. I. Kanner (1997)
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Analysis of the Transmembrane Topology of the Glycine Transporter GLYT1.
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Polarized Expression of GABA Transporters in Madin-Darby Canine Kidney Cells and Cultured Hippocampal Neurons.
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Science 257, 1271-1273
   Abstract »    PDF »
Cloning and expression of a cocaine-sensitive dopamine transporter complementary DNA.
S Shimada, S Kitayama, C. Lin, A Patel, E Nanthakumar, P Gregor, M Kuhar, and G Uhl (1991)
Science 254, 576-578
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Cloning and expression of a cocaine-sensitive rat dopamine transporter.
J. Kilty, D Lorang, and S. Amara (1991)
Science 254, 578-579
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Cloning of a serotonin transporter affected by antidepressants.
B. Hoffman, E Mezey, and M. Brownstein (1991)
Science 254, 579-580
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Mutation of Arginine 44 of GAT-1, a (Na+ + Cl-)-coupled gamma -Aminobutyric Acid Transporter from Rat Brain, Impairs Net Flux but Not Exchange.
E. R. Bennett, H. Su, and B. I. Kanner (2000)
J. Biol. Chem. 275, 34106-34113
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The Glial and the Neuronal Glycine Transporters Differ in Their Reactivity to Sulfhydryl Reagents.
M. J. Roux, R. Martinez-Maza, A. Le Goff, B. Lopez-Corcuera, C. Aragon, and S. Supplisson (2001)
J. Biol. Chem. 276, 17699-17705
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Functional Regulation of gamma -Aminobutyric Acid Transporters by Direct Tyrosine Phosphorylation.
R. M. Law, A. Stafford, and M. W. Quick (2000)
J. Biol. Chem. 275, 23986-23991
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Identification and characterization of an amino acid transporter expressed differentially in liver.
S. Gu, H. L. Roderick, P. Camacho, and J. X. Jiang (2000)
PNAS 97, 3230-3235
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