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Science 14 September 1990:
Vol. 249. no. 4974, pp. 1291 - 1293
DOI: 10.1126/science.2205003
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Articles

Science, Vol 249, Issue 4974, 1291-1293
Copyright © 1990 by American Association for the Advancement of Science

articles

Differentiation of mouse erythroleukemia cells enhanced by alternatively spliced c-myb mRNA

BL Weber, EH Westin, and MF Clarke

Department of Internal Medicine, University of Michigan School of Medicine, Ann Arbor 48109.

C-myb, the normal cellular homolog of the retroviral transforming gene v-myb, encodes a nuclear, transcriptional regulatory protein (p75c-myb). C-myb is involved in regulating normal human hematopoiesis, and inhibits dimethyl sulfoxide-induced differentiation of Friend murine erythroleukemia (F-MEL) cells. An alternately spliced c-myb mRNA encodes a truncated version of p75c-myb (mbm2) that includes the DNA binding region and nuclear localization signal present in the c-myb protein, but does not contain the transcriptional regulatory regions. Constitutive expression of mbm2, in contrast to c-myb, here resulted in enhanced differentiation of F-MEL cells. These data suggest that the c-myb protooncogene encodes alternately spliced mRNA species with opposing effects on differentiation.


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