Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 14 September 1990:
Vol. 249. no. 4974, pp. 1285 - 1288
DOI: 10.1126/science.2119055
Prev | Table of Contents | Next

Articles

Science, Vol 249, Issue 4974, 1285-1288
Copyright © 1990 by American Association for the Advancement of Science

articles

Site-specific gene expression in vivo by direct gene transfer into the arterial wall

EG Nabel, G Plautz, and GJ Nabel

Department of Internal Medicine, Howard Hughes Medical Institute, University of Michigan Medical Center, Ann Arbor 48109-0650.

A recombinant beta-galactosidase gene has been expressed in a specific arterial segment in vivo by direct infection with a murine amphotropic retroviral vector or by DNA transfection with the use of liposomes. Several cell types in the vessel wall were transduced, including endothelial and vascular smooth muscle cells. After retroviral infection, a recombinant reporter gene was expressed for at least 5 months, and no helper virus was detected. Recombinant gene expression achieved by direct retroviral infection or liposome-mediated DNA transfection was limited to the site of infection and was absent from liver, lung, kidney, and spleen. These results demonstrate that site-specific gene expression can be achieved by direct gene transfer in vivo and could be applied to the treatment of such human diseases as atherosclerosis or cancer.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Ex vivo electroporation as a potent new strategy for nonviral gene transfer into autologous vein grafts.
T. Yamaoka, Y. Yonemitsu, K. Komori, H. Baba, T. Matsumoto, T. Onohara, and Y. Maehara (2005)
Am J Physiol Heart Circ Physiol 289, H1865-H1872
   Abstract »    Full Text »    PDF »
Current status of catheter- and stent-based gene therapy.
F. Sharif, K. Daly, J. Crowley, and T. O'Brien (2004)
Cardiovasc Res 64, 208-216
   Abstract »    Full Text »    PDF »
Nitric Oxide Synthase Gene Transfer as a Tool to Study Biology of Endothelial Cells.
Z. S. Katusic, N. M. Caplice, and K. A. Nath (2003)
Arterioscler Thromb Vasc Biol 23, 1990-1994
   Abstract »    Full Text »    PDF »
Safety and Feasibility of Catheter-Based Local Intracoronary Vascular Endothelial Growth Factor Gene Transfer in the Prevention of Postangioplasty and In-Stent Restenosis and in the Treatment of Chronic Myocardial Ischemia: Phase II Results of the Kuopio Angiogenesis Trial (KAT).
M. Hedman, J. Hartikainen, M. Syvanne, J. Stjernvall, A. Hedman, A. Kivela, E. Vanninen, H. Mussalo, E. Kauppila, S. Simula, et al. (2003)
Circulation 107, 2677-2683
   Abstract »    Full Text »    PDF »
Targeted inhibition of Stat3 with a decoy oligonucleotide abrogates head and neck cancer cell growth.
P. L. Leong, G. A. Andrews, D. E. Johnson, K. F. Dyer, S. Xi, J. C. Mai, P. D. Robbins, S. Gadiparthi, N. A. Burke, S. F. Watkins, et al. (2003)
PNAS 100, 4138-4143
   Abstract »    Full Text »    PDF »
Vascular Gene Therapy: A Reality of the 21st Century.
B. S. Zuckerbraun and E. Tzeng (2002)
Arch Surg 137, 854-861
   Abstract »    Full Text »    PDF »
Trends in lipoplex physical properties dependent on cationic lipid structure, vehicle and complexation procedure do not correlate with biological activity.
M. E. Ferrari, D. Rusalov, J. Enas, and C. J. Wheeler (2001)
Nucleic Acids Res. 29, 1539-1548
   Abstract »    Full Text »    PDF »
Adenovirus-Mediated Gene Transfer to Ischemic Brain : Ischemic Flow Threshold for Transgene Expression.
H. Ooboshi, S. Ibayashi, J. Takada, H. Yao, T. Kitazono, and M. Fujishima (2001)
Stroke 32, 1043-1047
   Abstract »    Full Text »    PDF »
Gene Therapy for Cardiovascular Disease.
S. L. Meyerson, C. L. Skelly, M. A. Curi, and L. B. Schwartz (2000)
Seminars in Cardiothoracic and Vascular Anesthesia 4, 289-300
   Abstract »    PDF »
Biodistribution of adenoviral vector to nontarget tissues after local in vivo gene transfer to arterial wall using intravascular and periadventitial gene delivery methods.
M. O. HILTUNEN, M. P. TURUNEN, A.-M. TURUNEN, T. T. RISSANEN, M. LAITINEN, V.-M. KOSMA, and S. YLÄ-HERTTUALA (2000)
FASEB J 14, 2230-2236
   Abstract »    Full Text »
Local drug delivery: an emerging approach in the treatment of restenosis.
D. S Ettenson and E. R Edelman (2000)
Vascular Medicine 5, 97-102
   Abstract »    PDF »
Antisense and gene therapy to prevent restenosis.
A. Ehsan and M. J Mann (2000)
Vascular Medicine 5, 103-114
   Abstract »    PDF »
Local adenovirus-mediated transfer of C-type natriuretic peptide suppresses vascular remodeling in porcine coronary arteries in vivo.
K. Morishige, H. Shimokawa, T. Yamawaki, K. Miyata, Y. Eto, T. Kandabashi, K. Yogo, T. Higo, K. Egashira, H. Ueno, et al. (2000)
J. Am. Coll. Cardiol. 35, 1040-1047
   Abstract »    Full Text »    PDF »
Insights into the molecular pathogenesis of atherosclerosis and therapeutic strategies using gene transfer.
M. O Hiltunen, M. P Turunen, M. Laitinen, and S. Yla-Herttuala (2000)
Vascular Medicine 5, 41-48
   Abstract »    PDF »
Phase I Study of Direct Intralesional Gene Transfer of HLA-B7 into Metastatic Renal Carcinoma Lesions.
B. I. Rini, L. M. Selk, and N. J. Vogelzang (1999)
Clin. Cancer Res. 5, 2766-2772
   Abstract »    Full Text »    PDF »
Catheter-based myocardial gene transfer utilizing nonfluoroscopic electromechanical left ventricular mapping.
P. R. Vale, D. W. Losordo, T. Tkebuchava, D. Chen, C. E. Milliken, and J. M. Isner (1999)
J. Am. Coll. Cardiol. 34, 246-254
   Abstract »    Full Text »    PDF »
Retrovirus-mediated HO gene transfer into endothelial cells protects against oxidant-induced injury.
L. Yang, S. Quan, and N. G. Abraham (1999)
Am J Physiol Lung Cell Mol Physiol 277, L127-L133
   Abstract »    Full Text »    PDF »
Reporter Expression Persists 1 Year After Adeno-Associated Virus-Mediated Gene Transfer to the Optic Nerve.
J. Guy, X. Qi, N. Muzyczka, and W. W. Hauswirth (1999)
Arch Ophthalmol 117, 929-937
   Abstract »    Full Text »    PDF »
Endothelial nitric oxide synthase gene transfer enhances dilation of newborn piglet pulmonary arteries.
J. L. Aschner, N. Kovacs, J. V. Perciaccante, J. P. Figueroa, N. Thrikawala, G. S. Robins, and D. W. Busija (1999)
Am J Physiol Heart Circ Physiol 277, H371-H379
   Abstract »    Full Text »    PDF »
Pressure-mediated oligonucleotide transfection of rat and human cardiovascular tissues.
M. J. Mann, G. H. Gibbons, H. Hutchinson, R. S. Poston, E. G. Hoyt, R. C. Robbins, and V. J. Dzau (1999)
PNAS 96, 6411-6416
   Abstract »    Full Text »    PDF »
Liposomal Lipid and Plasmid DNA Delivery to B16/BL6 Tumors after Intraperitoneal Administration of Cationic Liposome DNA Aggregates.
D. L. Reimer, S. Kong, M. Monck, J. Wyles, P. Tam, E. K. Wasan, and M. B. Bally (1999)
J. Pharmacol. Exp. Ther. 289, 807-815
   Abstract »    Full Text »
High-efficiency gene transfer into skeletal muscle mediated by electric pulses.
L. M. Mir, M. F. Bureau, J. Gehl, R. Rangara, D. Rouy, J.-M. Caillaud, P. Delaere, D. Branellec, B. Schwartz, and D. Scherman (1999)
PNAS 96, 4262-4267
   Abstract »    Full Text »    PDF »
Molecular advances in cardiac and cardiovascular disease.
A. Basile-Borgia, J. H Abel, and H. Mahloogi (1999)
Perfusion 14, 89-99
   Abstract »    PDF »
Vascular Gene Transfer : From Bench to Bedside.
I. J. Kullo, R. D. Simari, and R. S. Schwartz (1999)
Arterioscler Thromb Vasc Biol 19, 196-207
   Full Text »    PDF »
Local Overexpression of Thrombomodulin for In Vivo Prevention of Arterial Thrombosis in a Rabbit Model.
J. M. Waugh, E. Yuksel, J. Li, M. D. Kuo, M. Kattash, R. Saxena, R. Geske, S. N. Thung, S. M. Shenaq, and S. L. C. Woo (1999)
Circ. Res. 84, 84-92
   Abstract »    Full Text »    PDF »
Apoptosis and Cell Proliferation After Porcine Coronary Angioplasty.
N. Malik, S. E. Francis, C. M. Holt, J. Gunn, G. L. Thomas, L. Shepherd, J. Chamberlain, C. M. H. Newman, D. C. Cumberland, and D. C. Crossman (1998)
Circulation 98, 1657-1665
   Abstract »    Full Text »    PDF »
Intracorporal injection of hSlo cDNA in rats produces physiologically relevant alterations in penile function.
G. J. Christ, J. Rehman, N. Day, L. Salkoff, M. Valcic, A. Melman, and J. Geliebter (1998)
Am J Physiol Heart Circ Physiol 275, H600-H608
   Abstract »    Full Text »    PDF »
Gene Therapy for Restenosis : Are We Ready?.
M. B. DeYoung and D. A. Dichek (1998)
Circ. Res. 82, 306-313
   Abstract »    Full Text »    PDF »
Optimal techniques for arterial gene transfer.
L. J Feldman and G. Steg (1997)
Cardiovasc Res 35, 391-404
   Abstract »    Full Text »    PDF »
Local drug delivery systems and prevention of restenosis.
D. Brieger and E. Topol (1997)
Cardiovasc Res 35, 405-413
   Full Text »    PDF »
Gene therapy for arterial thrombosis.
G. Vassalli and D. A Dichek (1997)
Cardiovasc Res 35, 459-469
   Abstract »    Full Text »    PDF »
Gene transfer into vascular cells using adeno-associated virus (AAV) vectors.
Y. Maeda, U. Ikeda, Y. Ogasawara, M. Urabe, T. Takizawa, T. Saito, P. Colosi, G. Kurtzman, K. Shimada, and K. Ozawa (1997)
Cardiovasc Res 35, 514-521
   Abstract »    Full Text »    PDF »
Downregulation of Cyclin G1 Expression by Retrovirus-Mediated Antisense Gene Transfer Inhibits Vascular Smooth Muscle Cell Proliferation and Neointima Formation.
N. L. Zhu, L. Wu, P. X. Liu, E. M. Gordon, W. F. Anderson, V. A. Starnes, and F. L. Hall (1997)
Circulation 96, 628-635
   Abstract »    Full Text »
Prevention of Restenosis by Local Drug Delivery.
R. R. Makkar, N. Eigler, F. Litvack, and J. S. Forrester (1996)
Journal of Cardiovascular Pharmacology and Therapeutics 1, 177-188
   Abstract »    PDF »
GENE THERAPY FOR DONOR HEARTS: EX VIVO LIPOSOME-MEDIATED TRANSFECTION.
J. Dalesandro, H. Akimoto, C. M. Gorman, T. O. McDonald, R. Thomas, H. D. Liggitt, and M. D. Allen (1996)
J. Thorac. Cardiovasc. Surg. 111, 416-422
   Abstract »    Full Text »
Expression and Function of a Recombinant PDGF B Gene in Porcine Arteries.
V. J. Pompili, D. Gordon, H. San, Z. Yang, D. W.M. Muller, G. J. Nabel, and E. G. Nabel (1995)
Arterioscler Thromb Vasc Biol 15, 2254-2264
   Abstract »    Full Text »
Efficiency of In Vivo Gene Transfection Into Transplanted Rat Heart by Coronary Infusion of HVJ Liposome.
Y. Sawa, K. Suzuki, H.-Z. Bai, R. Shirakura, R. Morishita, Y. Kaneda, and H. Matsuda (1995)
Circulation 92, 479-482
   Abstract »    Full Text »
Potential Gene Therapy Strategies in the Treatment of Cardiovascular Disease.
R. T. Rowland, J. C. Cleveland Jr, X. Meng, A. H. Harken, and J. M. Brown (1995)
Ann. Thorac. Surg. 60, 721-728
   Abstract »    Full Text »
Adenovirus-Mediated Gene Transfer In Vivo to Cerebral Blood Vessels and Perivascular Tissue.
H. Ooboshi, M. J. Welsh, C. D. Rios, B. L. Davidson, and D. D. Heistad (1995)
Circ. Res. 77, 7-13
   Abstract »    Full Text »
Direct gene transfer into donor hearts at the time of harvest.
A. Ardehali, A. Fyfe, H. Laks, D. C. Drinkwater Jr., J.-H. Qiao, and A. J. Lusis (1995)
J. Thorac. Cardiovasc. Surg. 109, 716-720
   Abstract »    Full Text »
Gene Therapy for Cardiovascular Disease.
E. G. Nabel (1995)
Circulation 91, 541-548
   Full Text »
Gene therapy for vascular smooth muscle cell proliferation after arterial injury.
T Ohno, D Gordon, H San, V. Pompili, M. Imperiale, G. Nabel, and E. Nabel (1994)
Science 265, 781-784
   Abstract »    PDF »
Gene Therapy and the Hemophilias.
J. N. Lozier and K. M. Brinkhous (1994)
JAMA 271, 47-51
   Abstract »    PDF »
Molecular Genetic Interventions for Cancer.
G.J. Nabel, E.G. Nabel, Z. Yang, B.A. Fox, G.E. Plautz, X. Gao, L. Huang, S. Shu, D. Gordon, and A.E. Chang (1994)
Cold Spring Harb Symp Quant Biol 59, 699-707
   Abstract »    PDF »
Systemic gene expression after intravenous DNA delivery into adult mice.
N Zhu, D Liggitt, Y Liu, and R Debs (1993)
Science 261, 209-211
   Abstract »    PDF »
Molecular advances in cardiovascular biology.
K. Chien (1993)
Science 260, 916-917
   PDF »
The basic science of gene therapy.
R. Mulligan (1993)
Science 260, 926-932
   Abstract »    PDF »
Platelet-Endothelium Interactions.
J. A. Ware and D. D. Heistad (1993)
N. Engl. J. Med. 328, 628-635
   Full Text »
Implanted myoblasts not only fuse with myofibers but also survive as muscle precursor cells.
S. Yao and K Kurachi (1993)
J. Cell Sci. 105, 957-963
   Abstract »    PDF »
Human Gene Therapy: A Role for the Primary Care Physician.
C. J. Schmeichel and J. M. Loeb (1992)
Arch Fam Med 1, 113-120
   Abstract »    PDF »
Systemic delivery of recombinant proteins by genetically modified myoblasts.
E Barr and J. Leiden (1991)
Science 254, 1507-1509
   Abstract »    PDF »
Systemic delivery of human growth hormone by injection of genetically engineered myoblasts.
J Dhawan, L. Pan, G. Pavlath, M. Travis, A. Lanctot, and H. Blau (1991)
Science 254, 1509-1512
   Abstract »    PDF »
Gene Therapy in Humans: A New Era Begins.
W. N. Kelley (1991)
Ann Intern Med 114, 697-698
   Abstract »    PDF »
DIRECTING GENE THERAPY TO THE ARTERIAL WALL.
(1990)
Journal Watch (General) 1990, 6
   Full Text »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)