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Science 31 August 1990:
Vol. 249. no. 4972, pp. 1049 - 1051
DOI: 10.1126/science.2144364
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Articles

Science, Vol 249, Issue 4972, 1049-1051
Copyright © 1990 by American Association for the Advancement of Science

articles

Transcriptional activation by wild-type but not transforming mutants of the p53 anti-oncogene

L Raycroft, HY Wu, and G Lozano

University of Texas, M. D. Anderson Cancer Center, Department of Molecular Genetics, Houston 77030.

The protein encoded by the wild-type p53 proto-oncogene has been shown to suppress transformation, whereas certain mutations that alter p53 become transformation competent. Fusion proteins between p53 and the GAL4 DNA binding domain were made to anchor p53 to a DNA target sequence and to allow measurement of transcriptional activation of a reporter plasmid. The wild-type p53 stimulated transcription in this assay, but two transforming mutations in p53 were unable to act as transcriptional activators. Therefore, p53 can activate transcription, and transformation-activating mutations result in a loss of function of the p53 protein. The inability of the p53 mutant proteins to activate transcription may enable them to be transformation competent.


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